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Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using PARP4 antibody.)

Rabbit PARP4 Polyclonal Antibody | anti-PARP4 antibody

PARP4 Polyclonal Antibody

Gene Names
PARP4; PH5P; p193; ARTD4; PARPL; VPARP; VWA5C; PARP-4; VAULT3; ADPRTL1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
PARP4; Polyclonal Antibody; PARP4 Polyclonal Antibody; PH5P; p193; ARTD4; PARPL; VPARP; VWA5C; PARP-4; VAULT3; ADPRTL1; anti-PARP4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PARP4 polyclonal antibody detects endogenous levels of PARP4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1724
Applicable Applications for anti-PARP4 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant protein of human PARP4.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using PARP4 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using PARP4 antibody.)
Related Product Information for anti-PARP4 antibody
This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
143
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 193kDa
NCBI Official Full Name
protein mono-ADP-ribosyltransferase PARP4
NCBI Official Synonym Full Names
poly(ADP-ribose) polymerase family member 4
NCBI Official Symbol
PARP4
NCBI Official Synonym Symbols
PH5P; p193; ARTD4; PARPL; VPARP; VWA5C; PARP-4; VAULT3; ADPRTL1
NCBI Protein Information
protein mono-ADP-ribosyltransferase PARP4
UniProt Protein Name
Poly [ADP-ribose] polymerase 4
UniProt Gene Name
PARP4
UniProt Synonym Gene Names
ADPRTL1; KIAA0177; PARPL; PARP-4; ARTD4; PH5P; VPARP
UniProt Entry Name
PARP4_HUMAN

NCBI Description

This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus. [provided by RefSeq, Jul 2008]

Uniprot Description

PARP4: Component of the vault ribonucleoprotein particle, at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1.

Protein type: EC 2.4.2.30; Transferase

Chromosomal Location of Human Ortholog: 13q11

Cellular Component: membrane; spindle microtubule; cytoplasm; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; NAD+ ADP-ribosyltransferase activity

Biological Process: cell death; response to drug; protein amino acid ADP-ribosylation; transport; protein modification process; inflammatory response; DNA repair; response to DNA damage stimulus

Research Articles on PARP4

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Product Notes

The PARP4 parp4 (Catalog #AAA3009132) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PARP4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PARP4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PARP4 parp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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