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Western Blot (WB) (Western Blot analysis of extracts of various cells, using SETD3 antibody.)

Rabbit anti-Human, Mouse SETD3 Polyclonal Antibody | anti-SETD3 antibody

SETD3 Polyclonal Antibody

Gene Names
SETD3; hSETD3; C14orf154
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SETD3; Polyclonal Antibody; SETD3 Polyclonal Antibody; C14orf154; Histone-lysine N-methyltransferase setd3; SET domain-containing protein 3; anti-SETD3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SETD3 polyclonal antibody detects endogenous levels of SETD3 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
594
Applicable Applications for anti-SETD3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1:50-1:100
Immunogen
A synthetic peptide of human SETD3.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cells, using SETD3 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of various cells, using SETD3 antibody.)
Related Product Information for anti-SETD3 antibody
SETD3 (SET domain containing 3) is a 594 amino acid protein that belongs to the SETD3 family. Containing one SET domain and localizing to nucleus, SETD3 is a histone methyltransferase that methylates Lys 36 of Histone H3. Overexpression of the setd3 protein in zebrafish decreases cell viability and activates caspase-3, indicating possible roles in apoptotic cell death and cell cycle regulation. In zebrafish the setd3 protein is composed of 17 alpha-helices and 8 beta-sheets in the secondary structures.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 67kDa
NCBI Official Full Name
actin-histidine N-methyltransferase isoform a
NCBI Official Synonym Full Names
SET domain containing 3, actin histidine methyltransferase
NCBI Official Symbol
SETD3
NCBI Official Synonym Symbols
hSETD3; C14orf154
NCBI Protein Information
actin-histidine N-methyltransferase
UniProt Protein Name
Histone-lysine N-methyltransferase setd3
UniProt Gene Name
SETD3
UniProt Synonym Gene Names
C14orf154
UniProt Entry Name
SETD3_HUMAN

Uniprot Description

SETD3: Histone methyltransferase that methylates 'Lys-36' of histone H3 (H3K36me). H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Belongs to the SETD3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.43; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 14q32.2

Cellular Component: nuclear chromatin

Molecular Function: histone lysine N-methyltransferase activity (H3-K4 specific); transcription coactivator activity; histone lysine N-methyltransferase activity (H3-K36 specific)

Biological Process: transcription, DNA-dependent; peptidyl-lysine di-methylation; positive regulation of transcription, DNA-dependent; peptidyl-lysine tri-methylation; histone H3-K4 methylation; peptidyl-lysine mono-methylation; positive regulation of muscle cell differentiation; histone H3-K36 methylation; positive regulation of transcription from RNA polymerase II promoter

Research Articles on SETD3

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Product Notes

The SETD3 setd3 (Catalog #AAA3009027) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SETD3 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SETD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1:50-1:100. Researchers should empirically determine the suitability of the SETD3 setd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SETD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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