Rabbit anti-Human Cleaved-MMP-15 Polyclonal Antibody | anti-MMP-15 antibody

Cleaved-MMP-15 (Y132) Polyclonal Antibody

Cat. Num. AAA3008448

• Gene Names: MMP15; MMP-15; MT2MMP; MTMMP2; SMCP-2; MT2-MMP
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cleaved-MMP-15; Polyclonal Antibody; Cleaved-MMP-15 (Y132) Polyclonal Antibody; Matrix metalloproteinase-15; MMP-15; Membrane-type matrix metalloproteinase 2; MT-MMP 2; MTMMP2; Membrane-type-2 matrix metalloproteinase; MT2-MMP; MT2MMP; SMCP-2; MMP15; anti-MMP-15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cleaved-MMP-15 (Y132) polyclonal antibody detects endogenous levels of Cleaved-MMP-15 (60 kDa) protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 669

• Applicable Applications for anti-MMP-15 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human MMP-15.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cleaved-MMP-15 (Y132) pAb at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Related Product Information for anti-MMP-15 antibody

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92 kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is downregulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression.

NCBI and Uniprot Product Information

• NCBI GI #: 4505211
• NCBI GeneID: 4324
• NCBI Accession #: NP_002419.1
• NCBI GenBank Nucleotide #: NP_002419.1
• UniProt Accession #: P51511
• Molecular Weight: ~ 61, 80kDa
• NCBI Official Full Name: matrix metalloproteinase-15 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 15
• NCBI Official Symbol: MMP15
• NCBI Official Synonym Symbols: MMP-15; MT2MMP; MTMMP2; SMCP-2; MT2-MMP
• NCBI Protein Information: matrix metalloproteinase-15
• UniProt Protein Name: Matrix metalloproteinase-15
• UniProt Gene Name: MMP15
• UniProt Synonym Gene Names: MMP-15; MT-MMP 2; MTMMP2; MT2-MMP; MT2MMP
• UniProt Entry Name: MMP15_HUMAN

NCBI Description

This gene encodes a member of the peptidase M10 family and membrane-type subfamily of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Members of this subfamily contain a transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. The encoded preproprotein is proteolytically processed to generate the mature protease. This protein may play a role in cancer progression. [provided by RefSeq, Jan 2016]

Uniprot Description

MMP15: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular matrix; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; protein modification process; proteolysis; response to estradiol stimulus