Western Blot (WB)
(Western Blot (WB) analysis of CARS polyclonal antibody)
Rabbit CARS Polyclonal Antibody | anti-CARS antibody
CARS Polyclonal Antibody
Gene Names
CARS1; CARS; CYSRS; MGC:11246
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
CARS; Polyclonal Antibody; CARS Polyclonal Antibody; Cysteine--tRNA ligase; cytoplasmic; Cysteinyl-tRNA synthetase; CysRS; anti-CARS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CARS polyclonal antibody detects endogenous levels of CARS protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
809
Applicable Applications for anti-CARS antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant full length Human CARS.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of CARS polyclonal antibody)
Western Blot (WB)
(Western Blot (WB) analysis of CARS polyclonal antibody)
Related Product Information for anti-CARS antibody
Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of tRNA by their cognate amino acid in the initial step of ribosome-dependent protein biosynthesis. Cytoplasmic CysRS (Cysteinyl-tRNA synthetase, cytoplasmic), also known as CARS, is a 748 amino acid member of the class-I aminoacyl-tRNA synthetase protein family. Cytoplasmic CysRS is a monomeric protein that binds one zinc ion per subunit for use as a cofactor. Cytoplasmic CysRS uses ATP to convert L-cysteine and tRNA(Cys) into ADP, a diphosphate and L-cysteinyl-tRNA(Cys). A chromosomal aberration of the gene that encodes Cytoplasmic CysRS is associated with inflammatory myofibroblastic tumors (IMTs). Cytoplasmic CysRS is expressed as two isoforms produced by alternative splicing events.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 85kDa
NCBI Official Full Name
cysteine--tRNA ligase, cytoplasmic isoform e
NCBI Official Synonym Full Names
cysteinyl-tRNA synthetase 1
NCBI Official Symbol
CARS1
NCBI Official Synonym Symbols
CARS; CYSRS; MGC:11246
NCBI Protein Information
cysteine--tRNA ligase, cytoplasmic
UniProt Protein Name
Cysteine--tRNA ligase, cytoplasmic
Protein Family
UniProt Gene Name
CARS
UniProt Synonym Gene Names
CysRS
UniProt Entry Name
SYCC_HUMAN
NCBI Description
This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]
Research Articles on CARS
Similar Products
Product Notes
The CARS cars (Catalog #AAA3007995) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CARS Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CARS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the CARS cars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
