Rabbit GIT1 Polyclonal Antibody | anti-GIT1 antibody

GIT1 Polyclonal Antibody

Cat. Num. AAA3007443

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: GIT1; Polyclonal Antibody; GIT1 Polyclonal Antibody; ARF GTPase-activating protein GIT1; ARF GAP GIT1; Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1; anti-GIT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GIT1 polyclonal antibody detects endogenous levels of GIT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 770

• Applicable Applications for anti-GIT1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 566-610 of Human GIT1
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GIT1 polyclonal antibody at 1:500 dilutionLane1:PC3 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Mouse (40ug) Lane4:The Testis tissue lysate of Rat (40ug) Lane5:A549 whole cell lysate (40ug))

Related Product Information for anti-GIT1 antibody

G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. GIT-1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology.

NCBI and Uniprot Product Information

• NCBI GI #: 146231998
• NCBI GeneID: 28964
• NCBI Accession #: NP_001078923.1
• NCBI GenBank Nucleotide #: NP_001078923.1
• UniProt Accession #: Q9Y2X7
• Molecular Weight: ~ 95kDa
• NCBI Official Full Name: ARF GTPase-activating protein GIT1 isoform 1
• NCBI Official Synonym Full Names: GIT ArfGAP 1
• NCBI Official Symbol: GIT1
• NCBI Protein Information: ARF GTPase-activating protein GIT1
• UniProt Protein Name: ARF GTPase-activating protein GIT1
• Protein Family: ARF GTPase-activating protein
• UniProt Gene Name: Git1
• UniProt Synonym Gene Names: ARF GAP GIT1
• UniProt Entry Name: GIT1_MOUSE

Uniprot Description

GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs).

Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: focal adhesion; membrane; cytoplasm; cytosol

Molecular Function: protein binding; zinc ion binding

Biological Process: axon guidance; ephrin receptor signaling pathway; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity