Rabbit anti-Human, Rat F5 Polyclonal Antibody | anti-F5 antibody

F5 Polyclonal Antibody

Cat. Num. AAA3007068

• Gene Names: F5; FVL; PCCF; THPH2; RPRGL1
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: F5; Polyclonal Antibody; F5 Polyclonal Antibody; Coagulation factor V; Activated protein C cofactor; Proaccelerin; labile factor; anti-F5 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: F5 polyclonal antibody detects endogenous levels of F5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 2224

• Applicable Applications for anti-F5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human F5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of F5 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:PC12 whole cell lysate)

Related Product Information for anti-F5 antibody

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and factors X, IX, V, and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation factor V (Factor V, FV, proaccelerin, labile factor) is a 2, 196 amino acid, single chain glycoprotein that is cleaved by thrombin to yield an active, Ca2+ dependent dimer. This heterodimer is essential to the blood coagulation cascade. Together with catalytic Factor Xa and Ca2+ on the surface of platelets or endothelial cells, Factor Va coordinates in a prothrombinase complex, which mediates proteolysis of prothrombin into active thrombin. Due to both the procoagulant properties of Factor V in coordinating proteolytic activation of thrombin, and anticoagulant properties as a cofactor to activated protein C (APC), which selectively destroys FVa and FXa, alterations at the Factor V locus can contribute to hemorrhagic diathesis or thrombosis, respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 105990535
• NCBI GeneID: 2153
• NCBI Accession #: NP_000121.2
• NCBI GenBank Nucleotide #: NP_000121.2
• UniProt Accession #: P12259
• Molecular Weight: ~ 252kDa
• NCBI Official Full Name: coagulation factor V preproprotein
• NCBI Official Synonym Full Names: coagulation factor V
• NCBI Official Symbol: F5
• NCBI Official Synonym Symbols: FVL; PCCF; THPH2; RPRGL1
• NCBI Protein Information: coagulation factor V
• UniProt Protein Name: Coagulation factor V
• Protein Family: Coagulation factor
• UniProt Gene Name: F5
• UniProt Entry Name: FA5_HUMAN

NCBI Description

This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]

Uniprot Description

factor V: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. Defects in F5 are the cause of factor V deficiency (FA5D); also known as Owren parahemophilia. It is an hemorrhagic diastesis. Defects in F5 are the cause of thrombophilia due to activated protein C resistance (THPH2). THPH2 is a hemostatic disorder due to defective degradation of factor Va by activated protein C. It is characterized by a poor anticoagulant response to activated protein C resulting in tendency to thrombosis. Defects in F5 are a cause of susceptibility to Budd- Chiari syndrome (BDCHS). A syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera. Defects in F5 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F5 are associated with susceptibility to pregnancy loss, recurrent, type 1 (RPRGL1). RPRGL1 is a common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the multicopper oxidase family.

Protein type: Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: Golgi apparatus; extracellular space; membrane; endoplasmic reticulum; extracellular region; plasma membrane; vesicle

Molecular Function: protein binding; copper ion binding; serine-type endopeptidase activity

Biological Process: platelet activation; platelet degranulation; blood circulation; proteolysis; blood coagulation

Disease: Thrombophilia Due To Activated Protein C Resistance; Budd-chiari Syndrome; Pregnancy Loss, Recurrent, Susceptibility To, 1; Stroke, Ischemic; Factor V Deficiency