Rabbit RAPGEF3 Polyclonal Antibody | anti-RAPGEF3 antibody

RAPGEF3 Polyclonal Antibody

Cat. Num. AAA3005841

• Gene Names: RAPGEF3; EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RAPGEF3; Polyclonal Antibody; RAPGEF3 Polyclonal Antibody; Rap guanine nucleotide exchange factor 3; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; cAMP-regulated guanine nucleotide exchange factor I; cAMP-GEFI; CGEF1; EPAC; EPAC1; anti-RAPGEF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAPGEF3 polyclonal antibody detects endogenous levels of RAPGEF3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 923

• Applicable Applications for anti-RAPGEF3 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; ICC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 799-844 of Human RAPGEF3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RAPGEF3 polyclonal antibody at 1:500 dilutionLane1:A549 cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-RAPGEF3 antibody

3', 5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac (Epac1, cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the Ras family GTPases Rap 1 and Rap 2 by promoting GTP binding in a cAMP-dependent manner. Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission. The human Epac gene maps to chromosome 12q13.11 with transcript being abundant in the kidney and heart. In situ hybridization indicates expression of Epac in adult rat brain and selective expression in neonatal brain, including septum and thalamus.

NCBI and Uniprot Product Information

• NCBI GI #: 317373593
• NCBI GeneID: 10411
• UniProt Accession #: O95398
• Molecular Weight: ~ 99kDa
• NCBI Official Full Name: Rap guanine nucleotide exchange factor 3
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor 3
• NCBI Official Symbol: RAPGEF3
• NCBI Official Synonym Symbols: EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
• NCBI Protein Information: rap guanine nucleotide exchange factor 3
• UniProt Protein Name: Rap guanine nucleotide exchange factor 3
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF3
• UniProt Synonym Gene Names: CGEF1; EPAC; EPAC1; EPAC 1; cAMP-GEFI
• UniProt Entry Name: RPGF3_HUMAN

Uniprot Description

Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, misc.; GEFs

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: membrane; plasma membrane; endomembrane system; cAMP-dependent protein kinase complex

Molecular Function: Rap guanyl-nucleotide exchange factor activity; protein binding; guanyl-nucleotide exchange factor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; platelet activation; signal transduction; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; cell proliferation; positive regulation of angiogenesis; cAMP-mediated signaling; positive regulation of stress fiber formation; energy reserve metabolic process; positive regulation of cAMP catabolic process; angiogenesis; blood coagulation; regulation of protein kinase activity; regulation of insulin secretion; Rap protein signal transduction