Human EPAC1 (Exchange Protein Directly Activated by cAMP 1) ELISA Kit | EPAC1 elisa kit

Human EPAC1 (Exchange Protein Directly Activated by cAMP 1) ELISA Kit

Cat. Num. AAA7607909

• Gene Names: RAPGEF3; EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
• Reactivity: Human
• Synonyms: EPAC1 (Exchange Protein Directly Activated by cAMP 1); Human EPAC1 (Exchange Protein Directly Activated by cAMP 1) ELISA Kit; EPAC1; EPAC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EPAC1. No significant cross-reactivity or interference between EPAC1 and analogues was observed.
• Sequence Length: 923

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for EPAC1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 317373593
• NCBI GeneID: 10411
• UniProt Accession #: O95398
• NCBI Official Full Name: Rap guanine nucleotide exchange factor 3
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor 3
• NCBI Official Symbol: RAPGEF3
• NCBI Official Synonym Symbols: EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
• NCBI Protein Information: rap guanine nucleotide exchange factor 3
• UniProt Protein Name: Rap guanine nucleotide exchange factor 3
• UniProt Gene Name: RAPGEF3
• UniProt Synonym Gene Names: CGEF1; EPAC; EPAC1; EPAC 1; cAMP-GEFI
• UniProt Entry Name: RPGF3_HUMAN

Uniprot Description

Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, misc.; GEFs

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: membrane; plasma membrane; endomembrane system; cAMP-dependent protein kinase complex

Molecular Function: Rap guanyl-nucleotide exchange factor activity; protein binding; guanyl-nucleotide exchange factor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; platelet activation; signal transduction; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; cell proliferation; positive regulation of angiogenesis; cAMP-mediated signaling; positive regulation of stress fiber formation; energy reserve metabolic process; positive regulation of cAMP catabolic process; angiogenesis; blood coagulation; regulation of protein kinase activity; regulation of insulin secretion; Rap protein signal transduction