Rabbit Crk2 Polyclonal Antibody | anti-Crk2 antibody

Crk2 (Phospho-Try221) Polyclonal Antibody

Cat. Num. AAA3005534

• Gene Names: CRK; p38; CRKII
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Crk2; Polyclonal Antibody; Crk2 (Phospho-Try221) Polyclonal Antibody; Adapter molecule crk; Proto-oncogene c-Crk; p38 CRK; CRK; CRKII; anti-Crk2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Crk2 (Try221) polyclonal antibody detects endogenous levels of Crk2 protein only when phosphorylated at Try221.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 204

• Applicable Applications for anti-Crk2 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Crk2 around the phosphorylation site of Tyrosine 221.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-Crk2 (Try221) pAb in paraffin-embedded human liver carcinoma tissue at 1:100.)

Related Product Information for anti-Crk2 antibody

Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 41327710
• NCBI GeneID: 1398
• NCBI Accession #: NP_005197.3
• NCBI GenBank Nucleotide #: NP_005197.3
• UniProt Accession #: P46108
• Molecular Weight: ~ 42kDa
• NCBI Official Full Name: adapter molecule crk isoform b
• NCBI Official Synonym Full Names: CRK proto-oncogene, adaptor protein
• NCBI Official Symbol: CRK
• NCBI Official Synonym Symbols: p38; CRKII
• NCBI Protein Information: adapter molecule crk
• UniProt Protein Name: Adapter molecule crk
• Protein Family: CDPK-related kinase
• UniProt Gene Name: CRK
• UniProt Entry Name: CRK_HUMAN

NCBI Description

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

CRK: an adaptor protein with an SH2-SH3-SH3 domain structure. Recruits cytoplasmic proteins through SH2-phospho-tyrosine interaction. Phosphorylated by Abl, IGF-IR and EGFR. Phosphorylation induces a change in intramolecular folding and SH2 interactions, causing its rapid dissociation from the tyrosine kinase complex.

Protein type: Adaptor/scaffold; Oncoprotein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cytoplasm; plasma membrane; cytosol; nucleus; actin cytoskeleton

Molecular Function: protein phosphorylated amino acid binding; protein binding; ephrin receptor binding; SH3/SH2 adaptor activity; SH2 domain binding

Biological Process: regulation of transcription from RNA polymerase II promoter; platelet activation; regulation of Rac protein signal transduction; nerve growth factor receptor signaling pathway; activation of MAPKK activity; regulation of actin cytoskeleton organization and biogenesis; insulin receptor signaling pathway; ephrin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation