CPI-17 blocking peptide

CPI-17 (K32) Peptide

Cat. Num. AAA3004068

• Gene Names: PPP1R14A; CPI17; CPI-17; PPP1INL
• Applications: Blocking
• Purity: Synthetic peptide CPI-17 (K32). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Synonyms: CPI-17; CPI-17 (K32) Peptide; Protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI17; PPP1INL; PPP1R14A; CPI-17 blocking peptide

• Specificity: This peptide can be used with studies using MBS3004067 CPI-17 (K32) pAb.
• Purity/Purification: Synthetic peptide CPI-17 (K32). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Form/Format: 1 mg/ml in DI water.
• Sequence Length: 120

• Applicable Applications for CPI-17 blocking peptide: Blocking
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Related Product Information for CPI-17 blocking peptide

CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.

NCBI and Uniprot Product Information

• NCBI GI #: 345441807
• NCBI GeneID: 94274
• NCBI Accession #: NP_001230876.1
• NCBI GenBank Nucleotide #: NP_001230876.1
• UniProt Accession #: Q96A00
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 14A isoform 2
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory inhibitor subunit 14A
• NCBI Official Symbol: PPP1R14A
• NCBI Official Synonym Symbols: CPI17; CPI-17; PPP1INL
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 14A
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 14A
• UniProt Gene Name: PPP1R14A
• UniProt Synonym Gene Names: CPI17; PPP1INL; CPI-17
• UniProt Entry Name: PP14A_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

PPP1R14A: a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity. Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2 ) concentration. Involved in the regulation of cell morphology, re-organisation of microfilaments, and cell spreading. Also expressed in human platelets and in brain where it may play a role in cerebellar long-term synaptic depression. Phosphorylation by ILK or Rho kinase enhances its ability to inhibit PP-1A.

Protein type: Protein phosphatase, regulatory subunit; Inhibitor

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm

Molecular Function: protein phosphatase inhibitor activity

Biological Process: regulation of phosphorylation; negative regulation of catalytic activity