Rabbit N4BP1 Polyclonal Antibody | anti-N4BP1 antibody

N4BP1 (Y415) Polyclonal Antibody

Cat. Num. AAA3003656

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: N4BP1; Polyclonal Antibody; N4BP1 (Y415) Polyclonal Antibody; NEDD4 binding protein 1; KIAA0615; anti-N4BP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: N4BP1 (Y415) polyclonal antibody detects endogenous levels of N4BP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 896

• Applicable Applications for anti-N4BP1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 391-440 of Human N4BP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of N4BP1  (Y415)  pAb at 1:1000 dilutionLane1:A2780 whole cell lysate (20ug) Lane2:A549 whole cell lysate (20ug) Lane3:HEK293 whole cell lysate (20ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of N4BP1 (Y415) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-N4BP1 antibody

Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73alpha, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73alpha for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73alpha and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.

NCBI and Uniprot Product Information

• NCBI GI #: 48928019
• NCBI Accession #: NP_694574.3
• NCBI GenBank Nucleotide #: NP_694574.3
• UniProt Accession #: O75113
• Molecular Weight: ~ 100kDa
• NCBI Official Full Name: NEDD4-binding protein 1
• UniProt Protein Name: NEDD4-binding protein 1
• Protein Family: NEDD4-binding protein
• UniProt Gene Name: N4BP1
• UniProt Synonym Gene Names: KIAA0615; N4BP1
• UniProt Entry Name: N4BP1_HUMAN

Uniprot Description

N4BP1: Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. Belongs to the N4BP1 family.

Protein type: Nucleolus; Unknown function

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: PML body; nucleolus

Molecular Function: protein binding

Biological Process: negative regulation of protein ubiquitination; negative regulation of proteasomal ubiquitin-dependent protein catabolic process