Rabbit BRCAA1 Polyclonal Antibody | anti-BRCAA1 antibody

BRCAA1 (D810) Polyclonal Antibody

Cat. Num. AAA3003207

• Gene Names: ARID4B; BCAA; BRCAA1; RBP1L1; SAP180; RBBP1L1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BRCAA1; Polyclonal Antibody; BRCAA1 (D810) Polyclonal Antibody; AT-rich interactive domain-containing protein 4B; ARID domain-containing protein 4B; 180 kDa Sin3-associated polypeptide; Sin3-associated polypeptide p180; Breast cancer-associated antigen BRCAA1; Histone deacetylase complex subunit SAP180; Retinoblastoma-binding protein 1-like 1; ARID4B; RBBP1L1; RBP1L1; SAP180; anti-BRCAA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BRCAA1 (D810) polyclonal antibody detects endogenous levels of BRCAA1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1312

• Applicable Applications for anti-BRCAA1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 776-832 of Human BRCAA1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BRCAA1 (D810) pAb at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:SK-OVCAR3 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of BRCAA1 (D810) pAb at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug))

Related Product Information for anti-BRCAA1 antibody

The breast cancer-associated antigen BRCAA1, also designated ARID domaincontaining protein 4B (ARID4B), Sin3-associated polypeptide p180 (SAP180), or Retinoblastoma-binding protein 1-like 1 (RBBP1L1 or RBP1L1), shares 37% amino acid identity and an identical antigen epitope, SSKKQKRSHK, with Rb binding protein 1. High expression of BRCAA1 mRNA has been found in human breast, lung, colon, pancreatic and ovarian cancers, as well as in normal testis. This expression pattern indicates that BRCAA1 can be uses as a molecular marker for a broad range of human malignancies. BRCAA1 also interacts with RBBP1/ARID4A and regulates genomic imprinting at the Prader-WIlli syndrome (PWS)/Angelman syndrome (AS) domain.

NCBI and Uniprot Product Information

• NCBI GI #: 332164768
• NCBI GeneID: 51742
• NCBI Accession #: NP_001193723.1
• NCBI GenBank Nucleotide #: NP_001193723.1
• UniProt Accession #: Q4LE39
• Molecular Weight: ~ 150kDa
• NCBI Official Full Name: AT-rich interactive domain-containing protein 4B isoform 1
• NCBI Official Synonym Full Names: AT-rich interaction domain 4B
• NCBI Official Symbol: ARID4B
• NCBI Official Synonym Symbols: BCAA; BRCAA1; RBP1L1; SAP180; RBBP1L1
• NCBI Protein Information: AT-rich interactive domain-containing protein 4B
• UniProt Protein Name: AT-rich interactive domain-containing protein 4B
• UniProt Gene Name: ARID4B
• UniProt Synonym Gene Names: BRCAA1; RBBP1L1; RBP1L1; SAP180; ARID domain-containing protein 4B
• UniProt Entry Name: ARI4B_HUMAN

NCBI Description

This gene encodes a protein with sequence similarity to retinoblastoma-binding protein-1. The encoded protein is a subunit of the histone deacetylase-dependant SIN3A transcriptional corepressor complex, which functions in diverse cellular processes including proliferation, differentiation, apoptosis, oncogenesis, and cell fate determination. The gene product is recognized by IgG antibody isolated from a breast cancer patient and appears to be a molecular marker associated with a broad range of human malignancies. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]