Rabbit NDFIP1 Polyclonal Antibody | anti-NDFIP1 antibody

NDFIP1 Polyclonal Antibody

Cat. Num. AAA3007664

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NDFIP1; Polyclonal Antibody; NDFIP1 Polyclonal Antibody; NEDD4 family-interacting protein 1; Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activating protein; N4WBP5; PSEC0192; PSEC0223; anti-NDFIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NDFIP1 polyclonal antibody detects endogenous levels of NDFIP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 221

• Applicable Applications for anti-NDFIP1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 170-220 of Human NDFIP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NDFIP1 polyclonal antibody at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Related Product Information for anti-NDFIP1 antibody

Nedd4 family interacting protein-1 (Ndfip1), also referred to as N4WBP5, is a member of a family of highly conserved proteins. It has three transmembrane domains in its carboxy terminal and two PY motifs in its amino terminal. Ndfip1 is a 221 amino acid, Golgi-associated protein that may play a role in Golgi structure and function. Ndfip1 binds the WW domains of a number of Nedd4 family members called HECT-type E3 ubiquitin ligases. Ndfip1 is strongly expressed in surviving neurons around a site of injury. This suggests that ubiquitination may be a possible survival strategy and Ndfip1 may act as a neuroprotective protein. Ndfip1 may also have an effect on the function of Itch, another E3 ubiquitin ligase. Expression of Ndfip1 and its association with Itch may be promoted by T cell activation. Mice lacking Ndfip1 exhibit inactivation of Itch and accumulation of JunB causing them to die prematurely with severe skin and lung inflammation.

NCBI and Uniprot Product Information

• NCBI GI #: 13386480
• NCBI Accession #: NP_085048.1
• NCBI GenBank Nucleotide #: NP_085048.1
• UniProt Accession #: Q9BT67
• Molecular Weight: ~ 24kDa
• NCBI Official Full Name: NEDD4 family-interacting protein 1
• UniProt Protein Name: NEDD4 family-interacting protein 1
• Protein Family: NEDD4 family-interacting protein
• UniProt Gene Name: NDFIP1
• UniProt Synonym Gene Names: N4WBP5
• UniProt Entry Name: NFIP1_HUMAN

Uniprot Description

NDFIP1: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: Golgi membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane; endosome membrane; cell cortex

Molecular Function: signal transducer activity; protein binding; WW domain binding

Biological Process: negative regulation of interleukin-4 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; cellular iron ion homeostasis; regulation of lymphocyte differentiation; regulation of myeloid leukocyte differentiation; signal transduction; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; negative regulation of T-helper 2 type immune response; positive regulation of protein ubiquitination; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of protein transport; regulation of isotype switching to IgG isotypes; negative regulation of transporter activity