Rabbit FGF 22 Polyclonal Antibody | anti-FGF22 antibody

FGF 22 (A96) Polyclonal Antibody

Cat. Num. AAA3003328

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FGF 22; Polyclonal Antibody; FGF 22 (A96) Polyclonal Antibody; Fibroblast growth factor 22; FGF-22; FGF22; UNQ2500/PRO5800; anti-FGF22 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FGF-22 (A96) polyclonal antibody detects endogenous levels of FGF-22 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 170

• Applicable Applications for anti-FGF22 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 64-112 of Human FGF 22.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of FGF 22 (A96) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of FGF 22 (A96) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of FGF 22 (A96) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-FGF22 antibody

Acidic and basic fibroblast growth factors (FGFs) are members of a family of multifunctional polypeptide growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Like other growth factors, FGFs act by binding and activating specific cell surface receptors. These receptors usually contain an extracellular ligand-binding region containing three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic tyrosine kinase domain. Fibroblast growth factor- 22 (FGF-22) is a secreted protein whose gene is localized to human chromosome 19p13.3. FGF-22 is expressed preferentially in the inner root sheath of the hair follicle, suggesting that it is important in hair development.

NCBI and Uniprot Product Information

• NCBI GI #: 10190672
• NCBI GeneID: 27006
• NCBI Accession #: NP_065688.1
• NCBI GenBank Nucleotide #: NP_065688.1
• UniProt Accession #: Q9HCT0
• Molecular Weight: ~ 20kDa
• NCBI Official Full Name: fibroblast growth factor 22 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor 22
• NCBI Official Symbol: FGF22
• NCBI Protein Information: fibroblast growth factor 22
• UniProt Protein Name: Fibroblast growth factor 22
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF22
• UniProt Synonym Gene Names: FGF-22
• UniProt Entry Name: FGF22_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The mouse homolog of this gene was found to be preferentially expressed in the inner root sheath of the hair follicle, which suggested a role in hair development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

FGF22: Plays a role in the fasting response, glucose homeostasis, lipolysis and lipogenesis. Can stimulate cell proliferation (in vitro). May be involved in hair development. Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; nucleolus; extracellular region

Molecular Function: growth factor activity; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; insulin receptor signaling pathway; innate immune response; cell differentiation