Rabbit Glucosidase IIbeta Polyclonal Antibody | anti-IIbeta antibody

Glucosidase IIbeta (K117) Polyclonal Antibody

Cat. Num. AAA3002884

• Gene Names: PRKCSH; GIIB; PCLD; PLD1; G19P1; PCLD1; PKCSH; AGE-R2; VASAP-60
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Glucosidase IIbeta; Polyclonal Antibody; Glucosidase IIbeta (K117) Polyclonal Antibody; Glucosidase 2 subunit beta; 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain; PKCSH; PRKCSH; G19P1; anti-IIbeta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Glucosidase IIbeta (K117) polyclonal antibody detects endogenous levels of Glucosidase IIbeta protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 525

• Applicable Applications for anti-IIbeta antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 90-130 of Human Glucosidase IIbeta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Glucosidase IIbeta (K117) pAb at 1:500 dilutionLane1:The Testis tissue lysate of Mouse (40ug) Lane2:The Testis tissue lysate of Rat (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug) Lane6:MCF-7 whole cell lysate (40ug))

Related Product Information for anti-IIbeta antibody

Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains alpha and beta subunits (Glucosidase IIalpha and Glucosidase IIbeta). The alpha and beta subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIalpha is the catalyitc core of the enzyme and can function independently of the beta subunit. The sequence of Glucosidase IIbeta encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIbeta is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIbeta was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIbeta retains the complete complex in the ER.

NCBI and Uniprot Product Information

• NCBI GI #: 48255891
• NCBI GeneID: 5589
• NCBI Accession #: NP_001001329.1
• NCBI GenBank Nucleotide #: NP_001001329.1
• UniProt Accession #: P14314
• Molecular Weight: ~ 59, 80kDa
• NCBI Official Full Name: glucosidase 2 subunit beta isoform 2
• NCBI Official Synonym Full Names: protein kinase C substrate 80K-H
• NCBI Official Symbol: PRKCSH
• NCBI Official Synonym Symbols: GIIB; PCLD; PLD1; G19P1; PCLD1; PKCSH; AGE-R2; VASAP-60
• NCBI Protein Information: glucosidase 2 subunit beta
• UniProt Protein Name: Glucosidase 2 subunit beta
• UniProt Gene Name: PRKCSH
• UniProt Synonym Gene Names: G19P1; PKCSH
• UniProt Entry Name: GLU2B_HUMAN

NCBI Description

This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

PRKCSH: Regulatory subunit of glucosidase II. Defects in PRKCSH are a cause of polycystic liver disease (PCLD). PCLD is an autosomal dominant disorder and is characterized by the presence of multiple liver cysts of biliary epithelial origin. PCLD is a distinct clinical and genetic entity that can occur independently from autosomal dominant polycystic kidney disease (ADPKD), which in a considerable but uncertain proportion of cases is associated with hepatic cysts. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; intracellular

Molecular Function: protein kinase C binding; phosphoprotein binding; calcium ion binding

Biological Process: cellular protein metabolic process; protein folding; innate immune response; protein amino acid N-linked glycosylation via asparagine; N-glycan processing; post-translational protein modification

Disease: Polycystic Liver Disease