Western Blot (WB)
(Western Blot (WB) analysis of NY-CO-1 (K914) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Rabbit NY-CO-1 Polyclonal Antibody | anti-NY-CO-1 antibody
NY-CO-1 (K914) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
NY-CO-1; Polyclonal Antibody; NY-CO-1 (K914) Polyclonal Antibody; Nuclear export mediator factor NEMF; Antigen NY-CO-1; Serologically defined colon cancer antigen 1; NEMF; SDCCAG1; anti-NY-CO-1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NY-CO-1 (K914) polyclonal antibody detects endogenous levels of NY-CO-1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1076
Applicable Applications for anti-NY-CO-1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 881-930 of Human NY-CO-1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of NY-CO-1 (K914) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of NY-CO-1 (K914) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of NY-CO-1 (K914) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of NY-CO-1 (K914) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Related Product Information for anti-NY-CO-1 antibody
Protein names: Nuclear export mediator factor NEMF; Serologically defined colon cancer antigen 1; Gene names: NEMF; SDCCAG1. Function: Plays a role in nuclear export. Subunit structure: Interacts (via its N-terminus) with XPO1. Subcellular location: Nucleus.Tissue specificity: Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis. Domain: The N-terminal domain contains a nuclear export signal. The C-terminal domain may interact with cargo proteins. Miscellaneous: Active in normal lung tissue, but is inactive in several lung cancer cell lines.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 110kDa
NCBI Official Full Name
nuclear export mediator factor NEMF isoform 1
UniProt Protein Name
Nuclear export mediator factor NEMF
UniProt Gene Name
NEMF
UniProt Synonym Gene Names
SDCCAG1
UniProt Entry Name
NEMF_HUMAN
Uniprot Description
SDCCAG1: Plays a role in nuclear export. Belongs to the NEMF family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear export
Chromosomal Location of Human Ortholog: 14q22
Cellular Component: nucleus
Biological Process: nuclear export
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Product Notes
The NY-CO-1 nemf (Catalog #AAA3002644) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NY-CO-1 (K914) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NY-CO-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NY-CO-1 nemf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NY-CO-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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