Rabbit MARK1/2/3/4 Polyclonal Antibody | anti-MARK1/2/3/4 antibody

MARK1/2/3/4 (G152) Polyclonal Antibody

Cat. Num. AAA3002512

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MARK1/2/3/4; Polyclonal Antibody; MARK1/2/3/4 (G152) Polyclonal Antibody; Adhesion G protein-coupled receptor F5; G-protein coupled receptor 116; ADGRF5; GPR116; KIAA0758; anti-MARK1/2/3/4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MARK1/2/3/4 pAb detects endogenous levels of MARK1/2/3/4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 795

• Applicable Applications for anti-MARK1/2/3/4 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-1:2000
• Immunogen: Synthetic peptide, corresponding to Human GPR116.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MARK1/2/3/4 (G152) pAb at 1:1000 dilutionLane1:The Brain tissue lysate of Rat (40ug) Lane2:The Heart tissue lysate of Mouse (5ug) Lane3:L02 whole cell lysate (30ug) Lane4:HEK293T whole cell lysate (30ug))

Related Product Information for anti-MARK1/2/3/4 antibody

Microtubule associated proteins regulate the stability of microtubules and control processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking. The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases was identified based on their ability to phosphorylate microtubule-associated proteins (MAPs) including tau, MAP2 and MAP4. MARK proteins phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics. In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimer's disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death. The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity.

NCBI and Uniprot Product Information

• NCBI GI #: 153791472
• NCBI Accession #: NP_061120.3
• NCBI GenBank Nucleotide #: NP_061120.3
• UniProt Accession #: Q9P0L2; Q7KZI7; P27448; Q96L34
• Molecular Weight: ~ 85kDa
• NCBI Official Full Name: serine/threonine-protein kinase MARK1 isoform 2
• UniProt Protein Name: Serine/threonine-protein kinase MARK1
• UniProt Gene Name: MARK1
• UniProt Synonym Gene Names: Par-1c; Par1c
• UniProt Entry Name: MARK1_HUMAN

Uniprot Description

MARK1: a member of the CAMKL family of protein kinases. The MARK group of kinases is apparently involved in the control of cell polarity, microtubule stability and cancer. MARK1 forms a tight association with Tau in neurofibrillary tangles in Alzheimer disease tissue.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.26; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; CAMKL family; MARK subfamily

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: microtubule cytoskeleton; cytoskeleton; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylserine binding; tau-protein kinase activity; magnesium ion binding; ATP binding

Biological Process: Wnt receptor signaling pathway; neuron migration; cytoskeleton organization and biogenesis; protein amino acid phosphorylation