Rabbit PFKP Polyclonal Antibody | anti-PFKP antibody

PFKP (Q365) Polyclonal Antibody

Cat. Num. AAA3002426

• Gene Names: PFKP; PFKF; PFK-C; PFK-P; ATP-PFK
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PFKP; Polyclonal Antibody; PFKP (Q365) Polyclonal Antibody; 6-phosphofructokinase type C; 6-phosphofructokinase; platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase; PFKF; PFK1; PFK 1; anti-PFKP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PFKP (Q365) polyclonal antibody detects endogenous levels of PFKP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 776

• Applicable Applications for anti-PFKP antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 350-400 of Human PFKP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PFKP (Q365) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PFKP (Q365) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PFKP (Q365) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PFKP (Q365) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PFKP (Q365) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-PFKP antibody

Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis. There are three isozymes: muscle-type, liver-type, and platelet-type. Platelet-type phosphofructokinase (PFKP) is expressed in various cell types. Research studies have shown that genetic variations in PFKP are associated with individuals born small for gestational age that are prone to obesity and diabetes later in adulthood.

NCBI and Uniprot Product Information

• NCBI GI #: 334191701
• NCBI GeneID: 5214
• NCBI Accession #: NP_001229268.1
• NCBI GenBank Nucleotide #: NP_001229268.1
• UniProt Accession #: Q01813
• Molecular Weight: ~ 85kDa
• NCBI Official Full Name: ATP-dependent 6-phosphofructokinase, platelet type isoform 2
• NCBI Official Synonym Full Names: phosphofructokinase, platelet
• NCBI Official Symbol: PFKP
• NCBI Official Synonym Symbols: PFKF; PFK-C; PFK-P; ATP-PFK
• NCBI Protein Information: ATP-dependent 6-phosphofructokinase, platelet type
• UniProt Protein Name: 6-phosphofructokinase type C
• Protein Family: ATP-dependent 6-phosphofructokinase
• UniProt Gene Name: PFKP
• UniProt Synonym Gene Names: PFKF; PFK-C
• UniProt Entry Name: K6PP_HUMAN

NCBI Description

This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

Uniprot Description

PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Kinase, other; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 10p15.3-p15.2

Cellular Component: membrane; nucleus; cytosol

Molecular Function: metal ion binding; protein complex binding; 6-phosphofructokinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process