Rabbit KAD1 Polyclonal Antibody | anti-KAD1 antibody

KAD1 polyclonal antibody

Cat. Num. AAA3001345

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: KAD1; Polyclonal Antibody; KAD1 polyclonal antibody; AK1; Adenylate kinase isoenzyme 1; AK 1; ATP-AMP transphosphorylase 1; Myokinase; anti-KAD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: AK1 Polyclonal Antibody detects endogenous levels of AK1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-KAD1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: IHC: 1/100-1/300; IF: 1/200-1/1000; ELISA: 1/5000
• Immunogen: The antiserum was produced against synthesized peptide derived from human KAD1. AA range: 101-150
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of HepG2 cells, using KAD1 Antibody . The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using KAD1 Antibody . The picture on the right is blocked with the synthesized peptide.)

Related Product Information for anti-KAD1 antibody

Background: adenylate kinase 1(AK1) Homo sapiens. This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

NCBI and Uniprot Product Information

• NCBI GI #: 4502011
• NCBI GeneID: 203
• NCBI Accession #: NP_000467.1
• NCBI GenBank Nucleotide #: NM_000476.2
• UniProt Accession #: P00568; Q9BVK9; Q9UQC7
• Molecular Weight: 21,635 Da
• NCBI Official Full Name: adenylate kinase isoenzyme 1
• NCBI Official Synonym Full Names: adenylate kinase 1
• NCBI Official Symbol: AK1
• NCBI Protein Information: adenylate kinase isoenzyme 1; AK 1; myokinase; ATP:AMP phosphotransferase; ATP-AMP transphosphorylase 1; adenylate monophosphate kinase
• UniProt Protein Name: Adenylate kinase isoenzyme 1
• UniProt Gene Name: AK1
• UniProt Synonym Gene Names: AK 1
• UniProt Entry Name: KAD1_HUMAN

NCBI Description

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq, Jul 2008]

Uniprot Description

AK1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency (HAAKD). Belongs to the adenylate kinase family.

Protein type: Kinase, other; EC 2.7.4.3; EC 2.7.4.6; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: cytoplasm; plasma membrane; cytosol; outer dense fiber

Molecular Function: nucleoside diphosphate kinase activity; adenylate kinase activity; ATP binding

Biological Process: ATP metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; ADP biosynthetic process; AMP metabolic process; cell cycle arrest

Disease: Adenylate Kinase Deficiency, Hemolytic Anemia Due To