Rabbit HIST1H2BC Polyclonal Antibody | anti-HIST1H2BC antibody

HIST1H2BC polyclonal antibody

Cat. Num. AAA3000098

• Gene Names: HIST1H2BA; STBP; H2BFU; TSH2B; bA317E16.3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HIST1H2BC; Polyclonal Antibody; HIST1H2BC polyclonal antibody; Histone H2B type 1-A; Histone H2B; testis; TSH2B.1; Htsh2b; Testis-specific histone H2B; H2BC1; HIST1H2BA; TSH2B; anti-HIST1H2BC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: HIST1H2BC polyclonal antibody detects endogenous levels of HIST1H2BC protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Applicable Applications for anti-HIST1H2BC antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Immunogen: Synthetic peptide, corresponding to Human HIST1H2BC.
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of HIST1H2BC polyclonal antibody at 1:500 dilutionLane1:SHSY5Y whole cell lysate(40ug)Lane2:C6 whole cell lysate(40ug)Lane3:PC3 whole cell lysate(40ug))

Related Product Information for anti-HIST1H2BC antibody

Background: The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. The p300/CBP histone acetyltransferases acetylate multiple lysine residues in the amino terminal tail of histone H2B (Lys5, 12, 15, and 20) at gene promoters during transcriptional activation. Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the access of DNA to various DNA-binding proteins. In addition, acetylation of specific lysine residues creates docking sites that facilitate recruitment of many transcription and chromatin regulatory proteins that contain a bromodomain, which binds to acetylated lysine residues. Histone H2B is mono-ubiquitinated at Lys120 during transcriptional activation by the RAD6 E2 protein in conjunction with the BRE1A/BRE1B E3 ligase (also known as RNF20/RNF40). Mono-ubiquitinated histone H2B Lys120 is associated with the transcribed region of active genes and stimulates transcriptional elongation by facilitating FACT-dependent chromatin remodeling. In addition, it is essential for subsequent methylation of histone H3 Lys4 and Lys79, two additional histone modifications that regulate transcriptional initiation and elongation. In response to metabolic stress, AMPK is recruited to responsive genes and phosphorylates histone H2B at Lys36, both at promoters and in transcribed regions of genes, and may regulate transcriptional elongation. In response to multiple apoptotic stimuli, histone H2B is phosphorylated at Ser14 by the Mst1 kinase. Upon induction of apoptosis, Mst1 is cleaved and activated by caspase-3, leading to global phosphorylation of histone H2B during chromatin condensation. Interestingly, histone H2B is rapidly phosphorylated at irradiation-induced DNA damage foci in mouse embryonic fibroblasts. In this case, phosphorylation at Ser14 is rapid, depends on prior phosphorylation of H2AX Ser139, and occurs in the absence of apoptosis, suggesting that Ser14 phosphorylation may have distinct roles in DNA-damage repair and apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 24586679
• NCBI GeneID: 255626
• NCBI Accession #: NP_733759.1
• NCBI GenBank Nucleotide #: NM_170610.2
• UniProt Accession #: Q96A08; P33778; P62807
• Molecular Weight: 127
• NCBI Official Full Name: histone H2B type 1-A
• NCBI Official Synonym Full Names: histone cluster 1, H2ba
• NCBI Official Symbol: HIST1H2BA
• NCBI Official Synonym Symbols: STBP; H2BFU; TSH2B; bA317E16.3
• NCBI Protein Information: histone H2B type 1-A; histone 1, H2ba; histone H2B, testis; testis-specific histone H2B; H2B histone family, member U, (testis-specific)
• UniProt Protein Name: Histone H2B type 1-A
• Protein Family: Histone
• UniProt Gene Name: HIST1H2BA
• UniProt Synonym Gene Names: TSH2B
• UniProt Entry Name: H2B1A_HUMAN