MYOG / Myogenin Recombinant Protein | MYOG recombinant protein

Human MYOG / Myogenin Recombinant Protein

• Gene Names: MYOG; MYF4; myf-4; bHLHc3
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: MYOG / Myogenin; Human MYOG / Myogenin Recombinant Protein; Myogenin; Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4; BHLHC3; MYF4; MYOG recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 224

• Applicable Applications for MYOG recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Interacts with SMARCA4/BRG1/BAF190A. Interacts (via C-terminal region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex (By similarity). Interacts with CSRP3.
• Entry Function: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 18765727
• NCBI GeneID: 4656
• NCBI Accession #: NP_002470.2
• NCBI GenBank Nucleotide #: NP_002470.2
• UniProt Accession #: P15173
• NCBI Official Full Name: myogenin
• NCBI Official Synonym Full Names: myogenin
• NCBI Official Symbol: MYOG
• NCBI Official Synonym Symbols: MYF4; myf-4; bHLHc3
• NCBI Protein Information: myogenin
• UniProt Protein Name: Myogenin
• Protein Family: Myosin
• UniProt Gene Name: MYOG
• UniProt Synonym Gene Names: BHLHC3; MYF4; bHLHc3; Myf-4
• UniProt Entry Name: MYOG_HUMAN

NCBI Description

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]