Human Myogenin ELISA Kit | MYOG elisa kit

Human Myogenin ELISA Kit

Cat. Num. AAA765394

• Gene Names: MYOG; MYF4; myf-4; bHLHc3
• Reactivity: Human
• Synonyms: Myogenin; Human Myogenin ELISA Kit; MYOG (Myogenin)/BHLHC3/MYF4/bHLHc3Myogenic factor-4/myogenin/Class C basic helix-loop-helix protein 3/myf-4/Myf-4/MYF4myogenin/Myogenic factor 4/MYOGENIN/myogenin (myogenic factor 4); MYOG elisa kit

• Reactivity: Human
• Sequence Length: 224

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MYOG elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MYOG antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MYOG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MYOG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MYOG can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 18765727
• NCBI GeneID: 4656
• NCBI Accession #: NP_002470.2
• NCBI GenBank Nucleotide #: NM_002479.5
• Molecular Weight: 25,037 Da
• NCBI Official Full Name: myogenin
• NCBI Official Synonym Full Names: myogenin (myogenic factor 4)
• NCBI Official Symbol: MYOG
• NCBI Official Synonym Symbols: MYF4; myf-4; bHLHc3
• NCBI Protein Information: myogenin
• UniProt Protein Name: Myogenin
• Protein Family: Myosin
• UniProt Gene Name: MYOG
• UniProt Synonym Gene Names: BHLHC3; MYF4; bHLHc3; Myf-4
• UniProt Entry Name: MYOG_HUMAN

NCBI Description

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]

Uniprot Description

myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q31-q41

Cellular Component: nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin DNA binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity

Biological Process: cell cycle; mRNA transcription from RNA polymerase II promoter; muscle cell differentiation; muscle cell fate commitment; myoblast differentiation; negative regulation of cell proliferation; negative regulation of glycolysis; ossification; positive regulation of muscle atrophy; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; regulation of satellite cell proliferation; response to denervation involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; response to gravity; response to muscle activity involved in regulation of muscle adaptation; skeletal muscle atrophy; skeletal muscle development; skeletal muscle fiber development; skeletal muscle regeneration; striated muscle atrophy