Rabbit Gastric Inhibitory Polypeptide ELISA Kit | GIP elisa kit

Rabbit Gastric Inhibitory Polypeptide ELISA Kit

Cat. Num. AAA28964

• Reactivity: Rabbit
• Synonyms: Gastric Inhibitory Polypeptide; Rabbit Gastric Inhibitory Polypeptide ELISA Kit; GIP elisa kit

• Reactivity: Rabbit
• Specificity: No significant cross-reactivity or interference between Rabbit GIP and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GIP concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit GIP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GIP and GIP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for GIP elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GIP concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit GIP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GIP and GIP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758436
• NCBI GeneID: 2695
• NCBI Accession #: NP_004114.1
• NCBI GenBank Nucleotide #: NM_004123.2
• UniProt Accession #: P09681; Q4VB42; Q6NTD3
• Molecular Weight: 17,108 Da
• NCBI Official Full Name: gastric inhibitory polypeptide preproprotein
• NCBI Official Synonym Full Names: gastric inhibitory polypeptide
• NCBI Official Symbol: GIP
• NCBI Protein Information: gastric inhibitory polypeptide; incretin hormone; glucose-dependent insulinotropic polypeptide
• UniProt Protein Name: Gastric inhibitory polypeptide
• Protein Family: Gastric inhibitory polypeptide
• UniProt Gene Name: GIP
• UniProt Synonym Gene Names: GIP
• UniProt Entry Name: GIP_HUMAN

NCBI Description

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

Uniprot Description

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.3-q22

Cellular Component: extracellular space; cell soma; endoplasmic reticulum lumen; extracellular region

Molecular Function: hormone activity

Biological Process: response to drug; response to peptide hormone stimulus; adult locomotory behavior; positive regulation of insulin secretion; female pregnancy; signal transduction; sensory perception of pain; response to lipid; response to amino acid stimulus; endocrine pancreas development; response to organic cyclic substance; memory; response to starvation; response to selenium ion; cellular protein metabolic process; response to glucose stimulus; response to axon injury; regulation of insulin secretion