Mouse Protein CYR61 ELISA Kit | Cyr61 elisa kit

Mouse Protein CYR61 ELISA Kit

Cat. Num. AAA2884542

• Gene Names: Cyr61; CCN1; Igfbp10; AI325051
• Reactivity: Mouse
• Synonyms: Protein CYR61; Mouse Protein CYR61 ELISA Kit; 3CH61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Cyr61; Ccn1; Igfbp10; Cyr61 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse Protein CYR61
• Sequence Length: 379

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.312-20 ng/mL
• Sensitivity: < 0.166 ng/mL
• Intra-assay Precision: <=7.8%
• Inter-assay Precision: <=9.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for Cyr61 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6753594
• NCBI GeneID: 16007
• NCBI Accession #: NP_034646.1
• NCBI GenBank Nucleotide #: NM_010516.2
• UniProt Accession #: P18406
• Molecular Weight: 41,709 Da
• NCBI Official Full Name: protein CYR61
• NCBI Official Synonym Full Names: cysteine rich protein 61
• NCBI Official Symbol: Cyr61
• NCBI Official Synonym Symbols: CCN1; Igfbp10; AI325051
• NCBI Protein Information: protein CYR61
• UniProt Protein Name: Protein CYR61
• Protein Family: Protein
• UniProt Gene Name: Cyr61
• UniProt Synonym Gene Names: Ccn1; Igfbp10; IBP-10; IGF-binding protein 10; IGFBP-10
• UniProt Entry Name: CYR61_MOUSE

Uniprot Description

CYR61: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Belongs to the CCN family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular region; proteinaceous extracellular matrix

Molecular Function: extracellular matrix binding; growth factor binding; heparin binding; insulin-like growth factor binding; integrin binding

Biological Process: cell adhesion; cell-cell adhesion; cell-cell signaling; chemotaxis; extracellular matrix organization and biogenesis; intussusceptive angiogenesis; negative regulation of apoptosis; osteoblast differentiation; positive regulation of apoptosis; positive regulation of BMP signaling pathway; positive regulation of caspase activity; positive regulation of cell differentiation; positive regulation of cell migration; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of cell growth; signal transduction