Rat CYR61 ELISA Kit | CYR61 elisa kit

Rat Protein CYR61 ELISA Kit

Cat. Num. AAA9425693

• Reactivity: Rat
• Synonyms: CYR61; Rat Protein CYR61 ELISA Kit; Protein CYR61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Cyr61; Ccn1; Igfbp10; CYR61 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural ratProtein CYR61. No significant cross-reactivity or interference was observed. Note?Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between ratProtein CYR61and all the analogues, therefore, cross reaction may still exist.

• Samples: Serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 0.31ng/mL -20.0ng/mL.
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYR61 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofratProtein CYR61concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein CYR61. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forProtein CYR61 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein CYR61, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein CYR61 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31542447
• NCBI GeneID: 83476
• NCBI Accession #: NP_112617.2
• NCBI GenBank Nucleotide #: NM_031327.2
• UniProt Accession #: Q9ES72
• Molecular Weight: 41,687 Da
• NCBI Official Full Name: protein CYR61
• NCBI Official Synonym Full Names: cysteine-rich, angiogenic inducer, 61
• NCBI Official Symbol: Cyr61
• NCBI Protein Information: protein CYR61
• UniProt Protein Name: Protein CYR61
• Protein Family: Protein
• UniProt Gene Name: Cyr61
• UniProt Synonym Gene Names: Ccn1; Igfbp10; IBP-10; IGF-binding protein 10; IGFBP-10

NCBI Description

an extracellular heparin binding protein involved in supporting smooth muscle cell adhesion, promoting cell migration, and enhancing growth factor-stimulated mitogenesis [RGD, Feb 2006]

Uniprot Description

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (). CYR61-mediated gene regulation is dependent on heparin-binding (). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 ().