Dog Peroxisome proliferator-activated receptor alpha ELISA Kit | PPARA elisa kit

Dog Peroxisome proliferator-activated receptor alpha ELISA Kit

Cat. Num. AAA288438

• Reactivity: Dog
• Synonyms: Peroxisome proliferator-activated receptor alpha; Dog Peroxisome proliferator-activated receptor alpha ELISA Kit; PPARA elisa kit

• Reactivity: Dog
• Specificity: This assay recognizes recombinant and natural dog PPAR-alpha. No significant cross-reactivity or interference was observed.
• Sequence Length: 468

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 78.0 pg/ml -5000 pg/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of dog PPAR-alpha concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPARA elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPAR-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PPAR-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PPAR-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PPAR-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50978780
• NCBI GeneID: 403654
• NCBI Accession #: NP_001003093.1
• NCBI GenBank Nucleotide #: NM_001003093.1
• UniProt Accession #: Q95N78
• Molecular Weight: 52,123 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor alpha
• NCBI Official Symbol: PPARA
• NCBI Protein Information: peroxisome proliferator-activated receptor alpha
• UniProt Protein Name: Peroxisome proliferator-activated receptor alpha
• Protein Family: Peroxisome proliferator-activated receptor
• UniProt Gene Name: PPARA
• UniProt Synonym Gene Names: NR1C1; PPAR-alpha
• UniProt Entry Name: PPARA_CANLF

Uniprot Description

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2 ().