Rat Serine protease inhibitor A3N ELISA Kit | Serpina3n elisa kit

Rat Serine protease inhibitor A3N ELISA Kit

Cat. Num. AAA288407

• Gene Names: Serpina3n; SPI3; Spin3; CPi-26; Spin2c; Spi-2.2
• Reactivity: Rat
• Synonyms: Serine protease inhibitor A3N; Rat Serine protease inhibitor A3N ELISA Kit; Serpina3n elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat Serpin A3N. No significant cross-reactivity or interference was observed.
• Sequence Length: 408

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.078 ng/mL
• Intra-Assay CV: <=4.6%
• Inter-Assay CV: <=8.0%
• Recovery: 94%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Serpina3n elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serpin A3N. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Serpin A3N and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serpin A3N, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Serpin A3N in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13928716
• NCBI GeneID: 24795
• NCBI Accession #: NP_113719.1
• NCBI GenBank Nucleotide #: NM_031531.1
• UniProt Accession #: P09006; Q03312
• Molecular Weight: 46,652 Da
• NCBI Official Full Name: serine protease inhibitor A3N
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade A, member 3N
• NCBI Official Symbol: Serpina3n
• NCBI Official Synonym Symbols: SPI3; Spin3; CPi-26; Spin2c; Spi-2.2
• NCBI Protein Information: serine protease inhibitor A3N
• UniProt Protein Name: Serine protease inhibitor A3N
• Protein Family: Serine protease inhibitor
• UniProt Gene Name: Serpina3n
• UniProt Synonym Gene Names: Spin2c; Serpin A3N; SPI-3
• UniProt Entry Name: SPA3N_RAT

NCBI Description

secreted acidic glycoprotein that may act as a serine protease inhibitor; expression is transiently induced during inflammation [RGD, Feb 2006]

Uniprot Description

SERPINA3: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular region; extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: inflammatory response; response to cytokine stimulus; response to lipopolysaccharide; response to peptide hormone stimulus