Mouse Laminin subunit beta-3 ELISA Kit | Lamb3 elisa kit

Mouse Laminin subunit beta-3 ELISA Kit

Cat. Num. AAA288210

• Reactivity: Mouse
• Synonyms: Laminin subunit beta-3; Mouse Laminin subunit beta-3 ELISA Kit; Lamb3 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Laminin subunit beta-3. No significant cross-reactivity or interference was observed.
• Sequence Length: 1172

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/ml
• Sensitivity: 39.19 pg/mL
• Intra-Assay CV: <=7.4%
• Inter-Assay CV: <=9.2%
• Recovery: 92%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Lamb3 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin subunit beta-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Laminin subunit beta-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Laminin subunit beta-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Laminin subunit beta-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 485050325
• NCBI GeneID: 16780
• NCBI Accession #: NP_001264857.1
• NCBI GenBank Nucleotide #: NM_001277928.1
• UniProt Accession #: Q61087; Q91V90
• Molecular Weight: 128,900 Da
• NCBI Official Full Name: laminin subunit beta-3
• NCBI Official Synonym Full Names: laminin, beta 3
• NCBI Official Symbol: Lamb3
• NCBI Protein Information: laminin subunit beta-3
• UniProt Protein Name: Laminin subunit beta-3
• Protein Family: Laminin
• UniProt Gene Name: Lamb3
• UniProt Entry Name: LAMB3_MOUSE

Uniprot Description

LAMB3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMB3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. Defects in LAMB3 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). GABEB is a non- lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: basement membrane; extracellular region; laminin-5 complex; proteinaceous extracellular matrix

Molecular Function: protein complex binding

Biological Process: brown fat cell differentiation; cell adhesion