Human Geminin (GMNN) ELISA Kit | GMNN elisa kit

Human Geminin (GMNN) ELISA Kit

Cat. Num. AAA287173

• Gene Names: GMNN; Gem; MGORS6
• Reactivity: Human
• Synonyms: Geminin (GMNN); Human Geminin (GMNN) ELISA Kit; Gem; RP3-369A17.3; geminin; GMNN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GMNN. No significant cross-reactivity or interference between Human GMNN and analogues was observed.
• Sequence Length: 209

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GMNN elisa kit

This assay employs a two-site sandwich ELISA to quantitate GMNN in samples. An antibody specific for GMNN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GMNN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GMNN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GMNN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 354681980
• NCBI GeneID: 51053
• NCBI Accession #: NP_001238918.1
• NCBI GenBank Nucleotide #: NM_001251989.1
• UniProt Accession #: O75496; Q9H1Z1; B3KMM8
• Molecular Weight: 23,565 Da
• NCBI Official Full Name: geminin
• NCBI Official Synonym Full Names: geminin, DNA replication inhibitor
• NCBI Official Symbol: GMNN
• NCBI Official Synonym Symbols: Gem; MGORS6
• NCBI Protein Information: geminin
• UniProt Protein Name: Geminin
• Protein Family: Geminin
• UniProt Gene Name: GMNN

NCBI Description

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]

Uniprot Description

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.