Human L-amino acid oxidase (LAO) ELISA Kit | LAO elisa kit
Human L-amino acid oxidase (LAO) ELISA Kit
Gene Names
IL4I1; LAO; FIG1; LAAO
Reactivity
Human
Synonyms
L-amino acid oxidase (LAO); Human L-amino acid oxidase (LAO) ELISA Kit; FIG1; Fig-1 protein; interleukin 4 induced protein 1; interleukin four induced 1; LAO elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human LAO. No significant cross-reactivity or interference between Human LAO and analogues was observed.
Sequence Length
589
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.625 ng/mL - 40 ng/mL
Sensitivity
0.31 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for LAO elisa kit
Intended Uses: For the quantitative detection of Human L-amino acid oxidase (LAO) concentration in serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LAO in Human serum, plasma, tissue homogenates. An antibody specific for LAO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAO present is bound by the immobilized antibody. After removing any unbound substances, a biotin- conjugated antibody specific for LAO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAO bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LAO in Human serum, plasma, tissue homogenates. An antibody specific for LAO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAO present is bound by the immobilized antibody. After removing any unbound substances, a biotin- conjugated antibody specific for LAO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAO bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,328 Da
NCBI Official Full Name
L-amino-acid oxidase isoform 2
NCBI Official Synonym Full Names
interleukin 4 induced 1
NCBI Official Symbol
IL4I1
NCBI Official Synonym Symbols
LAO; FIG1; LAAO
NCBI Protein Information
L-amino-acid oxidase
UniProt Protein Name
L-amino-acid oxidase
UniProt Gene Name
IL4I1
UniProt Synonym Gene Names
FIG1; LAAO; LAO; IL4-induced protein 1; hFIG1
NCBI Description
This gene encodes a protein with limited similarity to L-amino acid oxidase which contains the conserved amino acids thought to be involved in catalysis and binding of flavin adenine dinucleotide (FAD) cofactor. The expression of this gene can be induced by interleukin 4 in B cells, however, expression of transcripts containing the first two exons of the upstream gene is found in other cell types. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Uniprot Description
Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation ().
Research Articles on LAO
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Product Notes
The Human LAO il4i1 (Catalog #AAA286956) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA286956 ELISA Kit recognizes Human LAO. It is sometimes possible for the material contained within the vial of "L-amino acid oxidase (LAO), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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