IDH2 recombinant protein

Recombinant Human IDH2 Protein

Cat. Num. AAA286197

• Gene Names: IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• Applications: Western Blot, ELISA, SDS-Page
• Purity: > 95% as determined by SDS-PAGE
• Synonyms: IDH2; Recombinant Human IDH2 Protein; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH; IDH2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% as determined by SDS-PAGE
• Sequence Length: 400

• Applicable Applications for IDH2 recombinant protein: Western Blot (WB), ELISA (EIA), SDS-PAGE, Antigen

NCBI and Uniprot Product Information

• NCBI GI #: 583966148
• NCBI GeneID: 3418
• NCBI Accession #: NP_001276839.1
• NCBI GenBank Nucleotide #: NM_001289910.1
• UniProt Accession #: P48735; Q96GT3; B2R6L6; B4DFL2
• Molecular Weight: 12 kDa
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase (NADP(+)) 2, mitochondrial
• NCBI Official Symbol: IDH2
• NCBI Official Synonym Symbols: IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• NCBI Protein Information: isocitrate dehydrogenase [NADP], mitochondrial
• UniProt Protein Name: Isocitrate dehydrogenase [NADP], mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH2
• UniProt Synonym Gene Names: IDH
• UniProt Entry Name: IDHP_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

IDH2: a mitochondrial oxidoreductase that catalyzes the third step of the citric acid cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Can also use oxalosuccinate as a substrate. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH2 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. May tightly associate or interact with the pyruvate dehydrogenase complex. A somatic mutation resulting in the R172K conversion results in a neomorphic enzyme activity that converts isocitrate to R(-)-2-hydroxyglutarate (2HG) occurs in a significant percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Elevated levels of 2HG are also correlated with an elevated risk of malignant brain tumors.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.42; Mitochondrial; Other Amino Acids Metabolism - glutathione; Oxidoreductase

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: isocitrate dehydrogenase (NADP+) activity; magnesium ion binding

Biological Process: 2-oxoglutarate metabolic process; isocitrate metabolic process; tricarboxylic acid cycle

Disease: D-2-hydroxyglutaric Aciduria 2