Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein ELISA Kit | REG1A elisa kit

Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit

Cat. Num. AAA285689

• Gene Names: REG1A; P19; PSP; PTP; REG; ICRF; PSPS; PSPS1
• Reactivity: Human
• Synonyms: Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein; Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit; ICRF; MGC12447; P19; PSP; PSPS; PSPS1; PTP; REG; islet cells regeneration factor|islet of langerhans regenerating protein|lithostathine 1 alpha|pancreatic stone protein; secretory|pancreatic thread; REG1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human REG1A. No significant cross-reactivity or interference between Human REG1A and analogues was observed.
• Sequence Length: 166

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for REG1A elisa kit

Pancreatic stone protein is the major component of the protein matrix of calculi in patients suffering from chronic calcifying pancreatitis. Secretory pancreatic stone protein is a glycoprotein in the pancreatic secretion. This protein, called PSPS, occurs in multiple molecular forms due to posttranslational processing.
The abundance of PSPS in pancreatic juice (10 to 14% of total protein) suggests that it plays an important role in exocrine pancreatic function. In vitro experiments show that PSPS inhibits CaCO(3) crystal growth. Since in all the pancreatic secretions are supersaturated in calcium carbonate, the physiologic role of PSPS may be related to its inhibitory properties. This gene encodes a protein that is secreted by the exocrine pancreas.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate REG1A in samples. An antibody specific for REG1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREG1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 29725633
• NCBI GeneID: 5967
• NCBI Accession #: NP_002900.2
• NCBI GenBank Nucleotide #: NM_002909.4
• UniProt Accession #: P05451; P11379; Q4ZG28
• Molecular Weight: 18,731 Da
• NCBI Official Full Name: lithostathine-1-alpha
• NCBI Official Synonym Full Names: regenerating family member 1 alpha
• NCBI Official Symbol: REG1A
• NCBI Official Synonym Symbols: P19; PSP; PTP; REG; ICRF; PSPS; PSPS1
• NCBI Protein Information: lithostathine-1-alpha
• UniProt Protein Name: Lithostathine-1-alpha
• Protein Family: Lithostathine
• UniProt Gene Name: REG1A
• UniProt Synonym Gene Names: PSPS; PSPS1; REG; ICRF; REG; PSP; PTP; REG-1-alpha

NCBI Description

This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. [provided by RefSeq, Jul 2008]

Uniprot Description

REG1A: Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p12

Molecular Function: carbohydrate binding; growth factor activity

Biological Process: positive regulation of cell proliferation