Mouse Abnormal spindle-like microcephaly ELISA Kit | ASPM elisa kit

Mouse Abnormal spindle-like microcephaly-associated protein (ASPM) ELISA Kit

Cat. Num. AAA285256

• Gene Names: Aspm; Sha1; MCPH5; Calmbp1; D330028K02Rik
• Reactivity: Mouse
• Synonyms: Abnormal spindle-like microcephaly; Mouse Abnormal spindle-like microcephaly-associated protein (ASPM) ELISA Kit; Abnormal Spindle Like Microcephaly Associated Protein (ASPM); ASPM elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ASPM. No significant cross-reactivity or interference between Mouse ASPM and analogues was observed.
• Sequence Length: 3477

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ASPM elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ASPM in samples. An antibody specific for ASPM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyASPM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASPM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASPM bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 87298845
• NCBI GeneID: 12316
• NCBI Accession #: NP_033921.3
• NCBI GenBank Nucleotide #: NM_009791.4
• UniProt Accession #: Q8CJ27; O88482; Q4G1G9; Q8BJI8; Q8BKT4; A0A0G2JES1; B1ARM7
• Molecular Weight: 212,056 Da
• NCBI Official Full Name: abnormal spindle-like microcephaly-associated protein homolog
• NCBI Official Synonym Full Names: abnormal spindle microtubule assembly
• NCBI Official Symbol: Aspm
• NCBI Official Synonym Symbols: Sha1; MCPH5; Calmbp1; D330028K02Rik
• NCBI Protein Information: abnormal spindle-like microcephaly-associated protein homolog
• UniProt Protein Name: Abnormal spindle-like microcephaly-associated protein homolog
• Protein Family: Abnormal spindle-like microcephaly-associated protein
• UniProt Gene Name: Aspm
• UniProt Synonym Gene Names: Calmbp1; Sha1; Calmodulin-binding protein 1

Uniprot Description

ASPM: Probable role in mitotic spindle regulation and coordination of mitotic processes. May have a preferential role in regulating neurogenesis. Defects in ASPM are the cause of microcephaly primary type 5 (MCPH5); also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 1|1 E4-F

Cellular Component: cytoplasm; cytoskeleton; microtubule; microtubule minus-end; midbody; nucleus; spindle pole

Molecular Function: calmodulin binding

Biological Process: brain development; cell cycle; cell division; cerebral cortex development; developmental growth; forebrain neuroblast division; maintenance of centrosome location; male gonad development; negative regulation of asymmetric cell division; negative regulation of neuron differentiation; neuron migration; oogenesis; positive regulation of canonical Wnt signaling pathway; positive regulation of neuroblast proliferation; regulation of meiotic cell cycle; spermatogenesis; spindle assembly involved in meiosis; spindle localization; spindle organization and biogenesis