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Western Blot (WB) (Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Mouse spleen tissue lysate, Primary antibody: 124338(TSEN15 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Rabbit anti-Human, Mouse C1orf19 Polyclonal Antibody | anti-C1orf19 antibody

C1orf19 Rabbit pAb

Gene Names
TSEN15; PCH2F; sen15; C1orf19
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
C1orf19; Polyclonal Antibody; C1orf19 Rabbit pAb; sen15; anti-C1orf19 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-C1orf19 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
Immunogen
Full length fusion protein
Conjugation
Unconjugated
Molecular Function
This gene encodes a subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from tRNA precursors. Alternative splicing results in multiple transcript variants. There is a pseudogene of this gene on chromosome 17.
Modification
Unmodified
Cell Position
Nucleus
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Mouse spleen tissue lysate, Primary antibody: 124338(TSEN15 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Western Blot (WB) (Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Mouse spleen tissue lysate, Primary antibody: 124338(TSEN15 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 124338(TSEN15 Antibody) at dilution 1/85. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 124338(TSEN15 Antibody) at dilution 1/85. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 124338(TSEN15 Antibody) at dilution 1/85. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 124338(TSEN15 Antibody) at dilution 1/85. (Original magnification: ×200))
Product Categories/Family for anti-C1orf19 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
13,888 Da
NCBI Official Full Name
Homo sapiens tRNA splicing endonuclease 15 homolog (S. cerevisiae), mRNA
NCBI Official Synonym Full Names
tRNA splicing endonuclease subunit 15
NCBI Official Symbol
TSEN15
NCBI Official Synonym Symbols
PCH2F; sen15; C1orf19
NCBI Protein Information
tRNA-splicing endonuclease subunit Sen15
UniProt Protein Name
tRNA-splicing endonuclease subunit Sen15
UniProt Gene Name
TSEN15
UniProt Synonym Gene Names
C1orf19; SEN15; HsSEN15
UniProt Entry Name
SEN15_HUMAN

NCBI Description

This gene encodes a subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from tRNA precursors. Alternative splicing results in multiple transcript variants. There is a pseudogene of this gene on chromosome 17. [provided by RefSeq, Jul 2014]

Uniprot Description

TSEN15: Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3' end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Belongs to the SEN15 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: nucleolus

Molecular Function: tRNA-intron endonuclease activity

Biological Process: tRNA splicing; mRNA processing

Research Articles on C1orf19

Similar Products

Product Notes

The C1orf19 tsen15 (Catalog #AAA4754599) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C1orf19 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's C1orf19 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the C1orf19 tsen15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C1orf19, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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