Human Anti-neutrophil antibody (ANA) ELISA Kit | ANA elisa kit

Human Anti-neutrophil antibody (ANA) ELISA Kit

Cat. Num. AAA285066

• Gene Names: NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX
• Reactivity: Human
• Synonyms: Anti-neutrophil antibody (ANA); Human Anti-neutrophil antibody (ANA) ELISA Kit; ANA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ANA. No significant cross-reactivity or interference between Human ANA and analogues was observed.
• Sequence Length: 481

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/mL - 1000 pg/mL
• Sensitivity: 7.8 pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ANA elisa kit

Intended Uses: For the quantitative detection of Human Anti-neutrophil antibody (ANA) concentration in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ANA in Human serum, plasma. An antigen specific for ANA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANA present is bound by the immobilized antigen. After removing any unbound substances, a biotin-conjugated antigen specific for ANA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 299829294
• NCBI GeneID: 4688
• NCBI Accession #: NP_001177723.1
• NCBI GenBank Nucleotide #: NM_001190794.1
• Molecular Weight: 50,337 Da
• NCBI Official Full Name: neutrophil cytosol factor 2 isoform 2
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 2
• NCBI Official Symbol: NCF2
• NCBI Official Synonym Symbols: NCF-2; NOXA2; P67PHOX; P67-PHOX
• NCBI Protein Information: neutrophil cytosol factor 2
• UniProt Protein Name: Neutrophil cytosol factor 2
• Protein Family: Protein anachronism
• UniProt Gene Name: NCF2
• UniProt Synonym Gene Names: NOXA2; P67PHOX; NCF-2
• UniProt Entry Name: NCF2_HUMAN

NCBI Description

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Uniprot Description

p67phox: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Interacts with SYTL1 and RAC1. Interacts with NCF4. May interact with NOXO1. Belongs to the NCF2/NOXA1 family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: acrosome; cytoplasm; cytosol; NADPH oxidase complex; nucleolus

Molecular Function: electron carrier activity; protein binding; protein C-terminus binding; Rac GTPase binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cell redox homeostasis; cellular defense response; innate immune response; respiratory burst; small GTPase mediated signal transduction; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Ii