Rat ADAM metallopeptidase domain 17 ELISA Kit | ADAM17 elisa kit

Rat ADAM metallopeptidase domain 17 (ADAM17) ELISA Kit

Cat. Num. AAA284656

• Gene Names: Adam17; TACE
• Reactivity: Rat
• Synonyms: ADAM metallopeptidase domain 17; Rat ADAM metallopeptidase domain 17 (ADAM17) ELISA Kit; ADAM18; CD156B; CSVP; MGC71942; TACE; ADAM metallopeptidase domain 18|TNF-alpha converting enzyme|a disintegrin and metalloprotease domain 17|snake venom-like protease|tumor necrosis factor; alpha;; ADAM17 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat ADAM17. No significant cross-reactivity or interference between Rat ADAM17 and analogues was observed.
• Sequence Length: 824

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ADAM17 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ADAM17 in samples. An antibody specific for ADAM17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 9945330
• NCBI GeneID: 57027
• NCBI Accession #: NP_064702.1
• NCBI GenBank Nucleotide #: NM_020306.2
• UniProt Accession #: Q9Z1K9
• Molecular Weight: 93,017 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 17
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 17
• NCBI Official Symbol: Adam17
• NCBI Official Synonym Symbols: TACE
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 17
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 17
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: Adam17
• UniProt Synonym Gene Names: Tace; ADAM 17

NCBI Description

expression occurs in response to oxygen glucose deprivation, may play a role in inhibition of apoptosis via induction of TNF-alpha release [RGD, Feb 2006]

Uniprot Description

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described.

Protein type: EC 3.4.24.86; Membrane protein, integral; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 6q16

Cellular Component: actin cytoskeleton; apical plasma membrane; cell surface; cytoplasm; cytosol; extracellular space; focal adhesion; integral component of membrane; integral component of plasma membrane; intercellular junction; membrane; membrane raft; plasma membrane

Molecular Function: integrin binding; interleukin-6 receptor binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; Notch binding; PDZ domain binding; SH3 domain binding

Biological Process: B cell differentiation; cell adhesion; cell adhesion mediated by integrin; cell motility; defense response to Gram-positive bacterium; epidermal growth factor receptor signaling pathway; germinal center formation; integrin-mediated signaling pathway; membrane protein ectodomain proteolysis; negative regulation of apoptosis; negative regulation of neuron projection development; negative regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; Notch signaling pathway; PMA-inducible membrane protein ectodomain proteolysis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cellular component movement; positive regulation of chemokine production; positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle; positive regulation of epidermal growth factor-activated receptor activity; positive regulation of protein phosphorylation; positive regulation of T cell chemotaxis; positive regulation of transforming growth factor beta receptor signaling pathway; production of molecular mediator of acute inflammatory response; proteolysis; regulation of axon regeneration; regulation of mast cell apoptotic process; regulation of neuron migration; response to drug; response to high density lipoprotein stimulus; response to hypoxia; response to lipopolysaccharide; spleen development; T cell differentiation in thymus; wound healing, spreading of epidermal cells