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Rat 5-oxoprolinase ELISA Kit | OPLAH elisa kit

Rat 5-oxoprolinase (OPLAH) ELISA Kit

Reactivity
Rat
Synonyms
5-oxoprolinase; Rat 5-oxoprolinase (OPLAH) ELISA Kit; OPLAH elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat OPLAH. No significant cross-reactivity or interference between Rat OPLAH and analogues was observed.
Sequence Length
1288
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for OPLAH elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate OPLAH in samples. An antibody specific for OPLAH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyOPLAH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OPLAH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OPLAH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
137,720 Da
NCBI Official Full Name
5-oxoprolinase
NCBI Official Synonym Full Names
5-oxoprolinase (ATP-hydrolysing)
NCBI Official Symbol
Oplah
NCBI Protein Information
5-oxoprolinase
UniProt Protein Name
5-oxoprolinase
Protein Family
UniProt Gene Name
Oplah
UniProt Synonym Gene Names
5-OPase

NCBI Description

catalyzes cleavage of 5-oxo-L-proline to form L-glutamate and is coupled to the hydrolysis of ATP to ADP and inorganic phosphate [RGD, Feb 2006]

Uniprot Description

OPLAH: Catalyzes the cleavage of 5-oxo-L-proline to form L- glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. Defects in OPLAH are the cause of 5-oxoprolinase deficiency (OPLAHD). A disorder characterized by calcium oxalate/carbonate urolithiasis, and excessive urinary 5- oxo-L-proline. Affected individuals have recurrent episodes of vomiting, diarrhea, and abdominal pain. Belongs to the oxoprolinase family.

Protein type: EC 3.5.2.9; Hydrolase; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: cytosol

Molecular Function: 5-oxoprolinase (ATP-hydrolyzing) activity; ATP binding

Biological Process: glutathione metabolic process

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Product Notes

The Rat OPLAH oplah (Catalog #AAA283478) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283478 ELISA Kit recognizes Rat OPLAH. It is sometimes possible for the material contained within the vial of "5-oxoprolinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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