Human TNFAIP1 (Tumor necrosis factor, alpha-induced protein 1, endothelial) ELISA Kit | TNFAIP1 elisa kit

Human TNFAIP1 (Tumor necrosis factor, alpha-induced protein 1, endothelial) ELISA Kit

Cat. Num. AAA7608144

• Gene Names: TNFAIP1; B12; B61; EDP1; BTBD34; hBACURD2
• Reactivity: Human
• Synonyms: TNFAIP1 (Tumor necrosis factor; alpha-induced protein 1; endothelial); Human TNFAIP1 (Tumor necrosis factor; endothelial) ELISA Kit; BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; hBACURD2; BTB/POZ domain-containing protein TNFAIP1; Protein B12; Tumor necrosis factor; endothelial; TNFAIP1; BACURD2; EDP1; TNFAIP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNFAIP1. No significant cross-reactivity or interference between TNFAIP1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFAIP1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10863937
• NCBI GeneID: 7126
• NCBI Accession #: NP_066960.1
• NCBI GenBank Nucleotide #: NM_021137.4
• UniProt Accession #: Q13829; Q5TZQ1
• Molecular Weight: 36,204 Da
• NCBI Official Full Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• NCBI Official Synonym Full Names: tumor necrosis factor, alpha-induced protein 1 (endothelial)
• NCBI Official Symbol: TNFAIP1
• NCBI Official Synonym Symbols: B12; B61; EDP1; BTBD34; hBACURD2
• NCBI Protein Information: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; BTB/POZ domain-containing protein TNFAIP1
• UniProt Protein Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• Protein Family: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein
• UniProt Gene Name: TNFAIP1
• UniProt Synonym Gene Names: BACURD2; EDP1; hBACURD2
• UniProt Entry Name: BACD2_HUMAN

NCBI Description

This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFAIP1: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity. Belongs to the BACURD family.

Protein type: DNA replication; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17q22-q23

Cellular Component: cytoplasm; nucleolus; endosome

Molecular Function: protein domain specific binding; protein binding; ubiquitin-protein ligase activity; GTP-Rho binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell migration; embryonic development; negative regulation of Rho protein signal transduction; apoptosis; stress fiber formation; protein ubiquitination; immune response; DNA replication; protein homooligomerization; positive regulation of DNA replication