Mouse WD repeat-containing protein 82 ELISA Kit | WDR82 elisa kit

Mouse WD repeat-containing protein 82 (WDR82) ELISA Kit

Cat. Num. AAA282778

• Gene Names: Wdr82; CDW5/WDR82; 9430077D24Rik
• Reactivity: Mouse
• Synonyms: WD repeat-containing protein 82; Mouse WD repeat-containing protein 82 (WDR82) ELISA Kit; WDR82 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse WDR82. No significant cross-reactivity or interference between Mouse WDR82 and analogues was observed.
• Sequence Length: 313

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for WDR82 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WDR82 in samples. An antibody specific for WDR82 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDR82 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WDR82 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WDR82 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 148223079
• NCBI GeneID: 77305
• NCBI Accession #: NP_084172.1
• NCBI GenBank Nucleotide #: NM_029896.1
• UniProt Accession #: Q8BFQ4; Q8K2G5; Q8VEE8
• Molecular Weight: 35,079 Da
• NCBI Official Full Name: WD repeat-containing protein 82
• NCBI Official Synonym Full Names: WD repeat domain containing 82
• NCBI Official Symbol: Wdr82
• NCBI Official Synonym Symbols: CDW5/WDR82; 9430077D24Rik
• NCBI Protein Information: WD repeat-containing protein 82
• UniProt Protein Name: WD repeat-containing protein 82
• Protein Family: WD repeat-containing protein
• UniProt Gene Name: Wdr82
• UniProt Synonym Gene Names: Cdw5

Uniprot Description

WDR82: a WD repeat-containing protein. A regulatory component of the histone H3K4 methylatransferase SET1 complex implicated in tethering this complex to transcriptional start sites of active genes. Facilitates histone H3K4 methylation via recruitment of the SETD1A or SETD1B to phospho-S5 in the C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Component of the SET1 complex, composed of at least the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA, ¿B, or -C. Associated with multiple protein complexes including an RNA polymerase II complex, MLL3/MLL4 complex and a chaperonin-containing TCP1 complex. Interacts with CUL4B. Interacts with RBBP5 and SETD1B. Interacts with SETD1A (via RRM domain). Interacts with POLR2B. Interacts with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). SETD1A enhances its interaction with POLR2A. Interacts with PPP1R10/PNUTS. Interacts with PPP1CA in the presence of PPP1R10/PNUTS.

Protein type: Methyltransferase

Chromosomal Location of Human Ortholog: 9|9 F1

Cellular Component: chromatin; histone methyltransferase complex; nuclear chromosome, telomeric region; nucleolus; nucleus

Molecular Function: chromatin binding; histone methyltransferase activity (H3-K4 specific)

Biological Process: histone H3-K4 methylation