Rabbit anti-Human RUNX3 Polyclonal Antibody | anti-RUNX3 antibody

RUNX3 (D196) Polyclonal Antibody

Cat. Num. AAA3013836

• Gene Names: RUNX3; AML2; CBFA3; PEBP2aC
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RUNX3; Polyclonal Antibody; RUNX3 (D196) Polyclonal Antibody; Runt-related transcription factor 3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; AML2; CBFA3; PEBP2A3; anti-RUNX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RUNX3 (D196) polyclonal antibody detects endogenous levels of RUNX3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 429

• Applicable Applications for anti-RUNX3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 164-220 of Human RUNX3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RUNX3 (D196) pAb at 1:500 dilutionLane1:SGC7901 whole cell lysate (40ug) Lane2:A375 whole cell lysate (40ug) Lane3:HuT78 whole cell lysate (40ug))

Related Product Information for anti-RUNX3 antibody

RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX3 is expressed in cells of hematopoietic origin, including myeloid and B-cell lines and spleen. By playing a role in controlling the growth and differentiation of gastric epithelial cells, RUNX3 is a strong candidate as a gastric cancer tumor suppressor. Specifically, hypermethylation inactivates the gene encoding RUNX3. The detection of hypermethylation at multiple regions within the RUNX3 CpG island may aid in the identification and risk assessment of gastric cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 72534652
• NCBI GeneID: 864
• NCBI Accession #: NP_001026850.1
• NCBI GenBank Nucleotide #: NP_001026850.1
• UniProt Accession #: Q13761
• Molecular Weight: ~ 44kDa
• NCBI Official Full Name: runt-related transcription factor 3 isoform 1
• NCBI Official Synonym Full Names: RUNX family transcription factor 3
• NCBI Official Symbol: RUNX3
• NCBI Official Synonym Symbols: AML2; CBFA3; PEBP2aC
• NCBI Protein Information: runt-related transcription factor 3
• UniProt Protein Name: Runt-related transcription factor 3
• Protein Family: Runt-related transcription factor
• UniProt Gene Name: RUNX3
• UniProt Synonym Gene Names: AML2; CBFA3; PEBP2A3; CBF-alpha-3; PEA2-alpha C
• UniProt Entry Name: RUNX3_HUMAN

NCBI Description

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

AML2: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA- binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; transcription factor activity; ATP binding

Biological Process: peripheral nervous system neuron development; transcription from RNA polymerase II promoter; axon guidance; hair follicle morphogenesis; regulation of transcription, DNA-dependent; cell maturation; interferon-gamma production; chondrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; negative regulation of epithelial cell proliferation; negative regulation of cell cycle