Human Phosphatidylinositol-binding clathrin assembly protein ELISA Kit | PICALM elisa kit

Human Phosphatidylinositol-binding clathrin assembly protein (PICALM) ELISA Kit

Cat. Num. AAA282754

• Gene Names: PICALM; LAP; CALM; CLTH
• Reactivity: Human
• Synonyms: Phosphatidylinositol-binding clathrin assembly protein; Human Phosphatidylinositol-binding clathrin assembly protein (PICALM) ELISA Kit; PICALM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PICALM. No significant cross-reactivity or interference between Human PICALM and analogues was observed.
• Sequence Length: 640

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PICALM elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PICALM in samples. An antibody specific for PICALM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPICALM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PICALM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PICALM bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 56788368
• NCBI GeneID: 8301
• NCBI Accession #: NP_001008660.1
• NCBI GenBank Nucleotide #: NM_001008660.2
• UniProt Accession #: Q13492; O60700; Q4LE54; Q6GMQ6; Q86XZ9; B4DTM3; E9PN05; F8VPG7
• Molecular Weight: 70,037 Da
• NCBI Official Full Name: phosphatidylinositol-binding clathrin assembly protein isoform 2
• NCBI Official Synonym Full Names: phosphatidylinositol binding clathrin assembly protein
• NCBI Official Symbol: PICALM
• NCBI Official Synonym Symbols: LAP; CALM; CLTH
• NCBI Protein Information: phosphatidylinositol-binding clathrin assembly protein
• UniProt Protein Name: Phosphatidylinositol-binding clathrin assembly protein
• Protein Family: Phosphatidylinositol-binding clathrin assembly protein
• UniProt Gene Name: PICALM
• UniProt Synonym Gene Names: CALM

NCBI Description

This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

PICALM: Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Vesicle

Chromosomal Location of Human Ortholog: 11q14.2

Cellular Component: AP-2 adaptor complex; cell soma; cell surface; clathrin coat of coated pit; clathrin-coated endocytic vesicle; clathrin-coated pit; clathrin-coated vesicle; cytosol; early endosome; Golgi apparatus; intracellular membrane-bound organelle; intrinsic component of membrane; membrane; nucleus; perinuclear region of cytoplasm; postsynaptic membrane; presynaptic membrane; vesicle

Molecular Function: cadherin binding; clathrin binding; clathrin heavy chain binding; low-density lipoprotein receptor binding; phosphatidylinositol binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; Rab GTPase binding

Biological Process: axonogenesis; cell proliferation; clathrin coat assembly; dendrite morphogenesis; endosomal transport; hemopoiesis; iron ion homeostasis; learning and/or memory; negative regulation of receptor-mediated endocytosis; positive regulation of GTPase activity; positive regulation of transcription, DNA-templated; protein complex assembly; receptor internalization; receptor-mediated endocytosis; regulation of endocytosis; regulation of protein localization; synaptic vesicle maturation; transcytosis; vesicle organization and biogenesis; vesicle-mediated transport

Disease: Leukemia, Acute Myeloid