Bovine 39S ribosomal protein L10 ELISA Kit | MRPL10 elisa kit

Bovine 39S ribosomal protein L10, mitochondrial (MRPL10) ELISA Kit

Cat. Num. AAA282613

• Gene Names: MRPL10; L10mt; MRP-L10
• Reactivity: Bovine
• Synonyms: 39S ribosomal protein L10; Bovine 39S ribosomal protein L10; mitochondrial (MRPL10) ELISA Kit; MRPL10 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bovine MRPL10. No significant cross-reactivity or interference between Bovine MRPL10 and analogues was observed.

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MRPL10 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MRPL10 in samples. An antibody specific for MRPL10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPL10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPL10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPL10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 300798419
• NCBI GeneID: 515014
• NCBI Accession #: NP_001179324.1
• NCBI GenBank Nucleotide #: NM_001192395.1
• UniProt Accession #: Q3MHY7
• Molecular Weight: 22,802 Da
• NCBI Official Full Name: 39S ribosomal protein L10, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L10
• NCBI Official Symbol: MRPL10
• NCBI Official Synonym Symbols: L10mt; MRP-L10
• NCBI Protein Information: 39S ribosomal protein L10, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L10, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL10
• UniProt Synonym Gene Names: L10mt; MRP-L10

Uniprot Description

MRPL10: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Nov 2010]

Protein type: Mitochondrial; Ribosomal; Translation

Cellular Component: intracellular ribonucleoprotein complex; mitochondrial inner membrane; mitochondrial large ribosomal subunit; nucleoplasm

Molecular Function: RNA binding; structural constituent of ribosome

Biological Process: mitochondrial translation; ribosome biogenesis; translation