Human Putative methyltransferase NSUN5 ELISA Kit | NSUN5 elisa kit
Human Putative methyltransferase NSUN5 (NSUN5) ELISA Kit
Gene Names
NSUN5; NOL1; p120; NOL1R; NSUN5A; WBSCR20; WBSCR20A; p120(NOL1)
Reactivity
Human
Synonyms
Putative methyltransferase NSUN5; Human Putative methyltransferase NSUN5 (NSUN5) ELISA Kit; NSUN5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human NSUN5. No significant cross-reactivity or interference between Human NSUN5 and analogues was observed.
Sequence Length
435
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for NSUN5 elisa kit
NSUN5 encodes a member of the evolutionarily conserved NOL1/NOP2/Sun domain family. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. WBSCR20 encodes a novel protein expressed in skeletal muscle with similarity to p120 (NOL1), a 120-kDa proliferation-associated nucleolar antigen, a member of an evolutionarily conserved protein family. A highly similar putative gene, WBSCR20B, flanks the WBS deletion at the telomeric side. Hemizygous deletion of either of the novel genes might contribute to the growth retardation, the myopathy or the premature aging effects in the pathogenesis of WBS.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NSUN5 in samples. An antibody specific for NSUN5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNSUN5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NSUN5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NSUN5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NSUN5 in samples. An antibody specific for NSUN5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNSUN5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NSUN5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NSUN5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,478 Da
NCBI Official Full Name
probable 28S rRNA (cytosine-C(5))-methyltransferase isoform 3
NCBI Official Synonym Full Names
NOP2/Sun RNA methyltransferase family member 5
NCBI Official Symbol
NSUN5
NCBI Official Synonym Symbols
NOL1; p120; NOL1R; NSUN5A; WBSCR20; WBSCR20A; p120(NOL1)
NCBI Protein Information
probable 28S rRNA (cytosine-C(5))-methyltransferase
UniProt Protein Name
Probable 28S rRNA (cytosine-C(5))-methyltransferase
Protein Family
UniProt Gene Name
NSUN5
UniProt Synonym Gene Names
NSUN5A; WBSCR20; WBSCR20A; NOL1R
NCBI Description
This gene encodes a member of an evolutionarily conserved family of proteins that may function as methyltransferases. This gene is located in a larger region of chromosome 7 that is deleted in Williams-Beuren syndrome, a multisystem developmental disorder. There are two pseudogenes for this gene located in the same region of chromosome 7. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Uniprot Description
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 3782 in 28S rRNA.
Research Articles on NSUN5
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Product Notes
The Human NSUN5 nsun5 (Catalog #AAA282348) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282348 ELISA Kit recognizes Human NSUN5. It is sometimes possible for the material contained within the vial of "Putative methyltransferase NSUN5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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