Human Polymerase I and transcript release factor ELISA Kit | PTRF elisa kit

Human Polymerase I and transcript release factor (PTRF) ELISA Kit

Cat. Num. AAA282314

• Gene Names: CAVIN1; CGL4; PTRF; CAVIN; FKSG13; cavin-1
• Reactivity: Human
• Synonyms: Polymerase I and transcript release factor; Human Polymerase I and transcript release factor (PTRF) ELISA Kit; PTRF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PTRF. No significant cross-reactivity or interference between Human PTRF and analogues was observed.
• Sequence Length: 390

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PTRF elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PTRF in samples. An antibody specific for PTRF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTRF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTRF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTRF bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 42734430
• NCBI GeneID: 284119
• NCBI Accession #: NP_036364.2
• NCBI GenBank Nucleotide #: NM_012232.5
• UniProt Accession #: Q6NZI2; O00535; Q6GMY1; Q96H74; Q9BT85; Q9HAP4; B2RAW7
• Molecular Weight: 22,597 Da
• NCBI Official Full Name: caveolae-associated protein 1
• NCBI Official Synonym Full Names: caveolae associated protein 1
• NCBI Official Symbol: CAVIN1
• NCBI Official Synonym Symbols: CGL4; PTRF; CAVIN; FKSG13; cavin-1
• NCBI Protein Information: caveolae-associated protein 1; polymerase I and transcript release factor
• UniProt Protein Name: Caveolae-associated protein 1
• Protein Family: Polymerase I and transcript release factor
• UniProt Gene Name: CAVIN1

NCBI Description

This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009]

Uniprot Description

PTRF: Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex. Defects in PTRF are the cause of congenital generalized lipodystrophy type 4 (CGL4). It is a disorder characterized by the association of congenital generalized lipodystrophy with muscular dystrophy and cardiac anomalies. Congenital generalized lipodystrophy is characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Belongs to the PTRF/SDPR family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: caveola; cytoplasm; cytosol; endoplasmic reticulum; intracellular membrane-bound organelle; membrane raft; mitochondrion; nucleoplasm; nucleus; plasma membrane; protein complex

Molecular Function: identical protein binding; protein binding; RNA binding; rRNA primary transcript binding

Biological Process: positive regulation of cell motility; regulation of transcription, DNA-templated; rRNA transcription; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter

Disease: Lipodystrophy, Congenital Generalized, Type 4