DNA Binding Protein-7 (DBP-7) Recombinant Protein | CHD7 recombinant protein

DNA Binding Protein-7 (DBP-7), human recombinant

Cat. Num. AAA844932

• Gene Names: CHD7; CRG; HH5; IS3; KAL5
• Applications: SDS-Page
• Purity: >=99%
• Synonyms: DNA Binding Protein-7 (DBP-7); human recombinant; Chromodomain-helicase-DNA-binding protein 7 (CHD-7) (EC 3.6.4.12) (ATP-dependent helicase CHD7); DBP; CHD7 recombinant protein

• Host: E Coli
• Purity/Purification: >=99%
• Form/Format: Sterile filtered and lyophilized with no additives; Appearance: Lyophilized protein
• Sequence Length: 2997

• Applicable Applications for CHD7 recombinant protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Reconstitute in ddH?O to a concentration of 1.0 mg/ml. Aliquot and store at -20 degree C for future use. Repeated freeze/thaw cycles should be avoided.
• Endotoxin Level: <0.1 ng/mug
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CHD7 recombinant protein

Background: DNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete beta-barrel capped at the opening by a C-terminal alpha-helix; they bind to the minor groove of a DNA duplex via the triple-stranded beta-sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein-protein interactions. In vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.

NCBI and Uniprot Product Information

• NCBI GI #: 54112403
• NCBI GeneID: 55636
• NCBI Accession #: NP_060250.2
• NCBI GenBank Nucleotide #: NM_017780.3
• UniProt Accession #: Q9P2D1; Q05DI5; Q2TAN4; Q66K35; Q7Z6C0; Q7Z7Q2; Q9NXA0; Q9NXA3; D0VBA5; E9PNZ2
• Molecular Weight: 9.44 kDa
• NCBI Official Full Name: chromodomain-helicase-DNA-binding protein 7
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 7
• NCBI Official Symbol: CHD7
• NCBI Official Synonym Symbols: CRG; HH5; IS3; KAL5
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 7
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 7
• Protein Family: Chromodomain-helicase-DNA-binding protein
• UniProt Gene Name: CHD7
• UniProt Synonym Gene Names: KIAA1416; CHD-7
• UniProt Entry Name: CHD7_HUMAN

NCBI Description

This gene encodes a protein that contains several helicase family domains. Mutations in this gene have been found in some patients with the CHARGE syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

CHD-7: Probable transcription regulator. May interact with CTCF. Interacts with CHD8. Widely expressed in fetal and adult tissues. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; DNA-binding; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 8q12.2

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding; helicase activity; ATP binding

Biological Process: heart morphogenesis; limb development; central nervous system development; olfactory behavior; blood circulation; positive regulation of multicellular organism growth; palate development; regulation of growth hormone secretion; adult walking behavior; olfactory nerve development; embryonic hindlimb morphogenesis; female genitalia development; regulation of neurogenesis; sensory perception of sound; regulation of transcription, DNA-dependent; skeletal development; T cell differentiation; adult heart development; inner ear morphogenesis; transcription, DNA-dependent; in utero embryonic development; olfactory bulb development; cranial nerve development; semicircular canal morphogenesis; chromatin modification; genitalia development; retina development in camera-type eye; artery morphogenesis; cognition; nose development; rRNA processing

Disease: Charge Syndrome; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Hypogonadotropic Hypogonadism 5 With Or Without Anosmia