Mouse Zinc finger CCHC-type and RNA-binding motif-containing protein 1 ELISA Kit | ZCRB1 elisa kit
Mouse Zinc finger CCHC-type and RNA-binding motif-containing protein 1 (ZCRB1) ELISA Kit
Gene Names
Zcrb1; Madp-1; 2700088M22Rik
Reactivity
Mouse
Synonyms
Zinc finger CCHC-type and RNA-binding motif-containing protein 1; Mouse Zinc finger CCHC-type and RNA-binding motif-containing protein 1 (ZCRB1) ELISA Kit; ZCRB1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZCRB1. No significant cross-reactivity or interference between Mouse ZCRB1 and analogues was observed.
Sequence Length
217
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZCRB1 elisa kit
ZCRB1 contains a RNA recognition motif. It was identified as one of the protein components of U11/U12 snRNPs. This protein and many other U11/U12 snRNP proteins are highly conserved in organisms known to contain U12-type introns. These proteins have been shown to be essential for cell viability, suggesting the key roles in U12-type splicing.
The deduced 217-amino acid protein contains an RNA-binding motif, a CCHC-type zinc finger motif, and lysine- and glutamine-rich sequences. Database analysis suggested that ZCRB1 is conserved from plant to primate. Northern blot analysis of mouse tissues detected highest expression in heart and testis, lower expression in cerebellum, hindbrain, muscle, lung, and fat, and lowest expression in liver.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZCRB1 in samples. An antibody specific for ZCRB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZCRB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZCRB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZCRB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
The deduced 217-amino acid protein contains an RNA-binding motif, a CCHC-type zinc finger motif, and lysine- and glutamine-rich sequences. Database analysis suggested that ZCRB1 is conserved from plant to primate. Northern blot analysis of mouse tissues detected highest expression in heart and testis, lower expression in cerebellum, hindbrain, muscle, lung, and fat, and lowest expression in liver.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZCRB1 in samples. An antibody specific for ZCRB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZCRB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZCRB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZCRB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,455 Da
NCBI Official Full Name
zinc finger CCHC-type and RNA-binding motif-containing protein 1 isoform 2
NCBI Official Synonym Full Names
zinc finger CCHC-type and RNA binding motif 1
NCBI Official Symbol
Zcrb1
NCBI Official Synonym Symbols
Madp-1; 2700088M22Rik
NCBI Protein Information
zinc finger CCHC-type and RNA-binding motif-containing protein 1
UniProt Protein Name
Zinc finger CCHC-type and RNA-binding motif-containing protein 1
UniProt Gene Name
Zcrb1
UniProt Synonym Gene Names
U11/U12 snRNP 31 kDa protein
Uniprot Description
ZCRB1: Up-regulated by morphine. Down-regulated at 30-36 degrees Celsius while it is up-regulated at 39 degrees Celsius. Component of the U11/U12 snRNPs that are part of the U12- type spliceosome.
Protein type: RNA-binding
Chromosomal Location of Human Ortholog: 15|15 E3
Cellular Component: nucleus; spliceosomal complex; U12-type spliceosomal complex
Molecular Function: metal ion binding; nucleic acid binding; RNA binding; zinc ion binding
Biological Process: mRNA processing; mRNA splicing, via spliceosome; RNA splicing
