Human Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha ELISA Kit | PIP5K1A elisa kit

Human Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha (PIP5K1A) ELISA Kit

Cat. Num. AAA281793

• Reactivity: Human
• Synonyms: Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; Human Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha (PIP5K1A) ELISA Kit; PIP5K1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PIP5K1A. No significant cross-reactivity or interference between Human PIP5K1A and analogues was observed.
• Sequence Length: 562

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5 pg/mL - 4000 pg/mL
• Sensitivity: 31.25 pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PIP5K1A elisa kit

Intended Uses: For the quantitative detection of Human Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha (PIP5K1A) concentration in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PIP5K1A in Human serum, plasma. An antibody specific for PIP5K1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIP5K1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIP5K1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIP5K1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 208431774
• NCBI GeneID: 8394
• NCBI Accession #: NP_001129108.1
• NCBI GenBank Nucleotide #: NM_001135636.1
• UniProt Accession #: Q99755; Q99754; Q99756; A8K4Q0; B4DIN0
• Molecular Weight: 58,119 Da
• NCBI Official Full Name: phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 3
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 5-kinase type 1 alpha
• NCBI Official Symbol: PIP5K1A
• NCBI Protein Information: phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
• Protein Family: Phosphatidylinositol 4-phosphate 5-kinase
• UniProt Gene Name: PIP5K1A
• UniProt Synonym Gene Names: PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; PIP5KIalpha

Uniprot Description

PIP5K1A: Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Interacts with RAC1. Interacts with TUT1. Forms a complex with CDH1/E-cadherin, CTNNB1/beta-catenin and CTNND1 at the plasma membrane upon calcium stimulation. Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.68; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytosol; focal adhesion; lamellipodium; mRNA cleavage and polyadenylation specificity factor complex; nuclear speck; nucleoplasm; nucleus; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-phosphate 5-kinase activity; 1-phosphatidylinositol-4-phosphate 5-kinase activity; ATP binding; kinase binding; protein binding

Biological Process: actin cytoskeleton reorganization; cell migration; fibroblast migration; focal adhesion assembly; glycerophospholipid metabolic process; keratinocyte differentiation; phagocytosis; phosphatidylinositol biosynthetic process; phosphatidylinositol phosphorylation; phospholipid biosynthetic process; protein localization to plasma membrane; regulation of phosphatidylinositol 3-kinase signaling; signal transduction