Mouse X-ray repair cross-complementing protein 5 ELISA Kit | XRCC5 elisa kit
Mouse X-ray repair cross-complementing protein 5 (XRCC5) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XRCC5 in samples. An antibody specific for XRCC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXRCC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XRCC5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XRCC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
Uniprot Description
Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.
Protein type: DNA-binding; EC 3.6.1.-; EC 3.6.4.-; Helicase; Nuclear receptor co-regulator; Nucleolus; RNA-binding
Chromosomal Location of Human Ortholog: 1 C3|1 36.5 cM
Cellular Component: chromosome; cytoplasm; intracellular ribonucleoprotein complex; membrane; nonhomologous end joining complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus; plasma membrane; protein complex
Molecular Function: 5'-deoxyribose-5-phosphate lyase activity; ATP binding; ATP-dependent DNA helicase activity; damaged DNA binding; DNA binding; double-stranded DNA binding; double-stranded telomeric DNA binding; helicase activity; hydrolase activity; hydrolase activity, acting on acid anhydrides; nucleotide binding; protein binding; protein C-terminus binding; RNA binding; telomeric DNA binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding
Biological Process: activation of innate immune response; cell proliferation; cellular hyperosmotic salinity response; cellular response to DNA damage stimulus; cellular response to gamma radiation; cellular response to X-ray; DNA recombination; DNA repair; double-strand break repair; double-strand break repair via nonhomologous end joining; immune system process; innate immune response; leptotene; negative regulation of t-circle formation; negative regulation of transcription, DNA-dependent; positive regulation of neurogenesis; positive regulation of protein kinase activity; positive regulation of telomere maintenance via telomerase; regulation of smooth muscle cell proliferation; regulation of transcription, DNA-templated; telomere maintenance; transcription, DNA-dependent
Research Articles on XRCC5
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Product Notes
The Mouse XRCC5 xrcc5 (Catalog #AAA281325) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281325 ELISA Kit recognizes Mouse XRCC5. It is sometimes possible for the material contained within the vial of "X-ray repair cross-complementing protein 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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