Human Protein zer-1 homolog ELISA Kit | ZER1 elisa kit

Human Protein zer-1 homolog (ZER1) ELISA Kit

Cat. Num. AAA280804

• Gene Names: ZER1; ZYG; C9orf60; ZYG11BL
• Reactivity: Human
• Synonyms: Protein zer-1 homolog; Human Protein zer-1 homolog (ZER1) ELISA Kit; ZER1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ZER1. No significant cross-reactivity or interference between Human ZER1 and analogues was observed.
• Sequence Length: 766

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ZER1 elisa kit

Hzyg, an essential protein for cell division at the initial developmental stages of this species, and to a Drosophila melanogaster gene product which is likely to be involved in meiosis. Hzyg mRNA encodes a protein of 766 amino acids, 14% of which are leucine residues, with some being arranged in four leucine rich repeat motives usually involved in protein-protein interactions. Hzyg is encoded by a single gene, located on chromosome 9q32-q34.1, and transcribed as two mRNA: a 5 kb transcript strongly expressed in testis and skeletal muscle and barely detectable in other human tissues, and an abundant 3.1 kb mRNA detected only in the testis. The cell specific expression of Hzyg transcripts in testis, and the conservation of this gene among distant species, suggest that this protein may have an important role during meiosis.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZER1 in samples. An antibody specific for ZER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 33589814
• NCBI GeneID: 10444
• NCBI Accession #: NP_006327.2
• NCBI GenBank Nucleotide #: NM_006336.3
• UniProt Accession #: Q7Z7L7; O00156; Q5T272; Q5T273
• Molecular Weight: 88,169 Da
• NCBI Official Full Name: protein zer-1 homolog
• NCBI Official Synonym Full Names: zyg-11 related cell cycle regulator
• NCBI Official Symbol: ZER1
• NCBI Official Synonym Symbols: ZYG; C9orf60; ZYG11BL
• NCBI Protein Information: protein zer-1 homolog
• UniProt Protein Name: Protein zer-1 homolog
• Protein Family: Protein
• UniProt Gene Name: ZER1
• UniProt Synonym Gene Names: C9orf60; ZYG; ZYG11BL

NCBI Description

This gene encodes a subunit of an E3 ubiquitin ligase complex that may be involved in meiosis. The encoded protein contains three leucine-rich repeat motifs. [provided by RefSeq, Nov 2012]

Uniprot Description

ZYG11BL: Probably acts as target recruitment subunit in the E3 ubiquitin ligase complex ZER1-CUL2-Elongin BC. Belongs to the zyg-11 family.

Protein type: Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: Cul2-RING ubiquitin ligase complex

Molecular Function: ubiquitin-protein transferase activity

Biological Process: protein ubiquitination; regulation of ubiquitin-protein transferase activity