Mouse Zinc finger matrin-type protein 5 ELISA Kit | ZMAT5 elisa kit
Mouse Zinc finger matrin-type protein 5 (ZMAT5) ELISA Kit
Gene Names
Zmat5; D11Bwg0572e; D11Bwg1548e; 2610510L01Rik
Reactivity
Mouse
Synonyms
Zinc finger matrin-type protein 5; Mouse Zinc finger matrin-type protein 5 (ZMAT5) ELISA Kit; ZMAT5 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZMAT5. No significant cross-reactivity or interference between Mouse ZMAT5 and analogues was observed.
Sequence Length
170
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZMAT5 elisa kit
ZMAT5 Contains 1 C3H1-type zinc finger. Alternatively spliced transcript variants have been described. Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression.
Zinc fingers are small protein structural motifs that can coordinate one or more zinc ions to help stabilize their folds. They can be classified into several different structural families and typically function as interaction modules that bind DNA, RNA, proteins, or small molecules. The name "zinc finger" was originally coined to describe the hypothesized structure of the repeated unit in Xenopus laevis transcription factor IIIA.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZMAT5 in samples. An antibody specific for ZMAT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZMAT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZMAT5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZMAT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Zinc fingers are small protein structural motifs that can coordinate one or more zinc ions to help stabilize their folds. They can be classified into several different structural families and typically function as interaction modules that bind DNA, RNA, proteins, or small molecules. The name "zinc finger" was originally coined to describe the hypothesized structure of the repeated unit in Xenopus laevis transcription factor IIIA.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZMAT5 in samples. An antibody specific for ZMAT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZMAT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZMAT5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZMAT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,886 Da
NCBI Official Full Name
zinc finger matrin-type protein 5 isoform 1
NCBI Official Synonym Full Names
zinc finger, matrin type 5
NCBI Official Symbol
Zmat5
NCBI Official Synonym Symbols
D11Bwg0572e; D11Bwg1548e; 2610510L01Rik
NCBI Protein Information
zinc finger matrin-type protein 5
UniProt Protein Name
Zinc finger matrin-type protein 5
Protein Family
UniProt Gene Name
Zmat5
UniProt Synonym Gene Names
D11Bwg1548e; U11/U12 snRNP 20 kDa protein
Uniprot Description
ZMAT5: Component of the U11/U12 snRNPs that are part of the U12- type spliceosome. Not found in the major spliceosome.
Chromosomal Location of Human Ortholog: 11 A1|11 2.96 cM
Cellular Component: nucleoplasm; nucleus; spliceosomal complex; U12-type spliceosomal complex
Molecular Function: metal ion binding; nucleic acid binding; zinc ion binding
Biological Process: mRNA processing; RNA splicing
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Product Notes
The Mouse ZMAT5 zmat5 (Catalog #AAA280587) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280587 ELISA Kit recognizes Mouse ZMAT5. It is sometimes possible for the material contained within the vial of "Zinc finger matrin-type protein 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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